Team:DTU-Denmark-2
From 2011.igem.org
(Difference between revisions)
Line 23: | Line 23: | ||
The method applies long complementary overhangs on the PCR product as well as the destination vector. These overhangs can anneal each other to form a stable hybridization product. The overhangs on the PCR product are custom made and independent of restriction sites, which is very convenient when working with fungi and mammalian cells. | The method applies long complementary overhangs on the PCR product as well as the destination vector. These overhangs can anneal each other to form a stable hybridization product. The overhangs on the PCR product are custom made and independent of restriction sites, which is very convenient when working with fungi and mammalian cells. | ||
<br><br> | <br><br> | ||
- | We will develop a standardized cloning system, called | + | We will develop a standardized cloning system, called “Plug’n’Play with DNA”, where certain categories of biological parts can be gathered. This means that the parts in the form of pre-produced PCR-products are directly mixed with a vector, which makes assembly of an expression vector possible within a few hours. All the parts in the form of PCR-products will be distributed in microtiter plates directly ready for cloning. <br><br> Furthermore, the “Plug’n’Play” kit will contain a back-up where all parts are contained on a plasmid to ensure amplification of a mutation free template if needed. |
The simple and easy use of the system will be demonstrated by developing a reporter targeting system for the fungus Aspergillus niger. Furthermore, will we demonstrate its application in mammalian cells.</p> | The simple and easy use of the system will be demonstrated by developing a reporter targeting system for the fungus Aspergillus niger. Furthermore, will we demonstrate its application in mammalian cells.</p> | ||
Revision as of 09:21, 31 August 2011
Sponsored by Comments or questions to the team? Please Email us |