"
Team:Kyoto/Luminescence/Note
From 2011.igem.org
(Difference between revisions)
Grassfield (Talk | contribs) (→Aug 4th, 2011) |
Grassfield (Talk | contribs) (→Aug 4th, 2011) |
||
| Line 2: | Line 2: | ||
== Aug 4th, 2011 == | == Aug 4th, 2011 == | ||
| - | * We | + | * We got drosophila melanogaster(oregon-rs) in 3 tubes from [http://gcoe.biol.sci.kyoto-u.ac.jp/gcoe/eng/member/2008/07/fuse_naoyuki.php Mr. Fuse]. |
| - | * We | + | * We made [http://www.biol.se.tmu.ac.jp/fly/www/standard-medium.html standard medium of Tokyo Metropolitan Univ. for drosophila]. |
::{| class="wikitable" | ::{| class="wikitable" | ||
|+ [http://www.biol.se.tmu.ac.jp/fly/www/standard-medium.html Standard medium of Tokyo Metropolitan Univ. for drosophila] | |+ [http://www.biol.se.tmu.ac.jp/fly/www/standard-medium.html Standard medium of Tokyo Metropolitan Univ. for drosophila] | ||
| Line 22: | Line 22: | ||
# after autoclave, add propionic acid into it.■ | # after autoclave, add propionic acid into it.■ | ||
|} | |} | ||
| - | * We | + | * We dispensed the standard-medium into 3 bottles. |
Revision as of 08:43, 31 August 2011
This page is the note of Luminescence Group.
Aug 4th, 2011
- We got drosophila melanogaster(oregon-rs) in 3 tubes from [http://gcoe.biol.sci.kyoto-u.ac.jp/gcoe/eng/member/2008/07/fuse_naoyuki.php Mr. Fuse].
- We made [http://www.biol.se.tmu.ac.jp/fly/www/standard-medium.html standard medium of Tokyo Metropolitan Univ. for drosophila].
[http://www.biol.se.tmu.ac.jp/fly/www/standard-medium.html Standard medium of Tokyo Metropolitan Univ. for drosophila] Materials Methods - water 500mL
- dry yeast 20g
- corn flour 45g
- glucose 50g
- agalose 3.5g
- propionic acid 1.5mL
- Stir dry yeast and agalose with about two-thirds of water. Then, autoclave it.
- Stir corn flour and glucose with the remaining water.
- Stir 1 and 2, then autoclave it again.
- after autoclave, add propionic acid into it.■
- We dispensed the standard-medium into 3 bottles.
