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Team:Waterloo
From 2011.igem.org
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| + | Self excising ribozymes, also known as introns, if understood and manipulated effectively will become a useful molecular biology tool to make experiments easier. To make this system work, intron sequences are used to flank protein parts. In this way, when the intron sequences are randomly spliced out, different fusion proteins will be made based on the combination of how the protein parts come together. This system is tested by using a simple GFP fusion protein. An intron sequence with an embedded lox site is flanked on either side by two components of GFP. The intron self-excises out so that the two GFP components that flank it are expressed to form a fully functional GFP protein. Given that this system works, it can be applied to a larger number of components to make more complicated expression systems. | ||
|[[Image:Waterloo_team.png|right|frame|Your team picture]] | |[[Image:Waterloo_team.png|right|frame|Your team picture]] | ||
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Revision as of 20:02, 30 August 2011
Ribozyme Project
The Ribozyme Project is the primary project for the Waterloo 2011 iGEM Team. It consists of a self-excising intron sequence which will provide a novel way to make fusion proteins without a ligation scar. For more information, please click on the links below to view the project description, safety, and up to date notebook entries.
| You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing. | File:Waterloo logo.png 200px |
Project AbstractSelf excising ribozymes, also known as introns, if understood and manipulated effectively will become a useful molecular biology tool to make experiments easier. To make this system work, intron sequences are used to flank protein parts. In this way, when the intron sequences are randomly spliced out, different fusion proteins will be made based on the combination of how the protein parts come together. This system is tested by using a simple GFP fusion protein. An intron sequence with an embedded lox site is flanked on either side by two components of GFP. The intron self-excises out so that the two GFP components that flank it are expressed to form a fully functional GFP protein. Given that this system works, it can be applied to a larger number of components to make more complicated expression systems. | File:Waterloo team.png Your team picture |
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