Team:Freiburg/Notebook/30 August

From 2011.igem.org

(Difference between revisions)
(→Precipitator)
(→Precipitator)
Line 39: Line 39:
'''Investigators: Sophie'''
'''Investigators: Sophie'''
<br/> Project name: inducible promoter for pbd <br/>
<br/> Project name: inducible promoter for pbd <br/>
-
again cloning of IPTG-inducible Promoter, new psb1C3-PCR product and ε5 (GFP-pbd) but this time I use Antarctic Phosphatase in the last hour of the digestion of the vector to avoid religation. For 50μL reaction volume use 5,6μL Antarctic Phosphatase buffer and 1μL Antarctic Phosphatase.
+
again cloning of IPTG-inducible Promoter, new psb1C3-PCR product and ε5 (GFP-pbd) but this time I use Antarctic Phosphatase in the last hour of the digestion of the vector to avoid re-ligation. For 50μL reaction volume use 5,6μL Antarctic Phosphatase buffer and 1μL Antarctic Phosphatase.
{{:Team:Freiburg/Templates/footer}}
{{:Team:Freiburg/Templates/footer}}

Revision as of 15:39, 30 August 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME


blue light receptor

Transformation

...with ♥-A3-Not, ♥-A3-nOt and ♥-A3-noT </br>

Investigators: Sophie

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

Cloning

Investigators: Sophie
Project name: inducible promoter for pbd
again cloning of IPTG-inducible Promoter, new psb1C3-PCR product and ε5 (GFP-pbd) but this time I use Antarctic Phosphatase in the last hour of the digestion of the vector to avoid re-ligation. For 50μL reaction volume use 5,6μL Antarctic Phosphatase buffer and 1μL Antarctic Phosphatase.

                       

Retrieved from "http://2011.igem.org/Team:Freiburg/Notebook/30_August"