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Team:Freiburg/Notebook/30 August
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==<span style="color:grey;">Precipitator</span>== | ==<span style="color:grey;">Precipitator</span>== | ||
| - | === | + | ===Cloning=== |
'''Investigators: Sophie''' | '''Investigators: Sophie''' | ||
| + | <br/> Project name: inducible promoter for pbd <br/> | ||
again cloning of IPTG-inducible Promoter, new psb1C3-PCR product and ε5 (GFP-pbd) but this time I use Antarctic Phosphatase in the last hour of the digestion of the vector to avoid religation. For 50μL reaction volume use 5,6μL Antarctic Phosphatase buffer and 1μL Antarctic Phosphatase. | again cloning of IPTG-inducible Promoter, new psb1C3-PCR product and ε5 (GFP-pbd) but this time I use Antarctic Phosphatase in the last hour of the digestion of the vector to avoid religation. For 50μL reaction volume use 5,6μL Antarctic Phosphatase buffer and 1μL Antarctic Phosphatase. | ||
{{:Team:Freiburg/Templates/footer}} | {{:Team:Freiburg/Templates/footer}} | ||
Revision as of 15:38, 30 August 2011
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This is the wiki page of the Freiburger student team competing for iGEM 2011. Thank you for your interest!
