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Copenhagen/29 August 2011
From 2011.igem.org
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(Created page with "==Monday== '''Labwork''' * PCR purification of B1 after prefix-suffix-PCR. The purifactions are Nanodropped - B1 samples 3,4,5,8 and 10 looks fine. * Digestions (1 h by 37 de...") |
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* Digestions (1 h by 37 degrees or O/N) | * Digestions (1 h by 37 degrees or O/N) | ||
| - | + | * PSB1C3 with E + P | |
| - | PSB1C3 with E + P | + | * A2 in PSB1C3 with X + P |
| - | + | * Expr.vector with P + S | |
| - | A2 in PSB1C3 with X + P | + | * B1 3,4,5,7 and 10 with E + P |
| - | + | ||
| - | Expr.vector with P + S | + | |
| - | + | ||
| - | B1 3,4,5,7 and 10 with E + P | + | |
* Purification of the digestions by using the PCR purifaction kit | * Purification of the digestions by using the PCR purifaction kit | ||
* Ligations | * Ligations | ||
| - | + | * PSB1C3 + B1 samples 3,4,5,8 and 10 | |
| - | PSB1C3 + B1 samples 3,4,5,8 and 10 | + | * Expr. vector + A2 |
| - | + | * Selfmade Expr. vector + A2 | |
| - | Expr. vector + A2 | + | |
| - | + | ||
| - | Selfmade Expr. vector + A2 | + | |
* Transformation of ligations | * Transformation of ligations | ||
Revision as of 15:21, 30 August 2011
Monday
Labwork
- PCR purification of B1 after prefix-suffix-PCR. The purifactions are Nanodropped - B1 samples 3,4,5,8 and 10 looks fine.
- Digestions (1 h by 37 degrees or O/N)
* PSB1C3 with E + P * A2 in PSB1C3 with X + P * Expr.vector with P + S * B1 3,4,5,7 and 10 with E + P
- Purification of the digestions by using the PCR purifaction kit
- Ligations
* PSB1C3 + B1 samples 3,4,5,8 and 10 * Expr. vector + A2 * Selfmade Expr. vector + A2
- Transformation of ligations
- Made O/N cultures on: 2C9, UserB1 to Sequencing, UserB1 to growth and Control BL21 cells to growth.
- Made TB media and autoclaved bottles for tomorrow.
- Made Cam + IPTG plates
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