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Copenhagen/29 August 2011
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Revision as of 15:18, 30 August 2011
Monday
Labwork
- PCR purification of B1 after prefix-suffix-PCR. The purifactions are Nanodropped - B1 samples 3,4,5,8 and 10 looks fine.
- Digestions (1 h by 37 degrees or O/N)
PSB1C3 with E + P
A2 in PSB1C3 with X + P
Expr.vector with P + S
B1 3,4,5,7 and 10 with E + P
- Purification of the digestions by using the PCR purifaction kit
- Ligations
PSB1C3 + B1 samples 3,4,5,8 and 10
Expr. vector + A2
Selfmade Expr. vector + A2
- Transformation of ligations
- Made O/N cultures on: 2C9, UserB1 to Sequencing, UserB1 to growth and Control BL21 cells to growth.
- Made TB media and autoclaved bottles for tomorrow.
- Made Cam + IPTG plates
Back to notebook
