Copenhagen/29 August 2011

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Revision as of 15:18, 30 August 2011

Monday

Labwork

  • PCR purification of B1 after prefix-suffix-PCR. The purifactions are Nanodropped - B1 samples 3,4,5,8 and 10 looks fine.
  • Digestions (1 h by 37 degrees or O/N)

PSB1C3 with E + P

A2 in PSB1C3 with X + P

Expr.vector with P + S

B1 3,4,5,7 and 10 with E + P

  • Purification of the digestions by using the PCR purifaction kit
  • Ligations

PSB1C3 + B1 samples 3,4,5,8 and 10

Expr. vector + A2

Selfmade Expr. vector + A2

  • Transformation of ligations
  • Made O/N cultures on: 2C9, UserB1 to Sequencing, UserB1 to growth and Control BL21 cells to growth.
  • Made TB media and autoclaved bottles for tomorrow.
  • Made Cam + IPTG plates


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