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Team:Bielefeld-Germany/HPLC isocratic
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* UV-detection at 227 nm | * UV-detection at 227 nm | ||
* Column: | * Column: | ||
| - | ** Eurospher II 100-5 C18p | + | ** Eurospher II 100-5 C18p by [http://www.knauer.net/ Knauer] |
** Dimensions: 150 x 4.6 mm with precolumn | ** Dimensions: 150 x 4.6 mm with precolumn | ||
** Particle size: 5 µm | ** Particle size: 5 µm | ||
** Pore size: 100 Å | ** Pore size: 100 Å | ||
** Material: silica gel | ** Material: silica gel | ||
Revision as of 12:58, 29 August 2011
HPLC method
- C18 reverse phase column
- Isocratic method: 45 % acetonitrile
- Flow = 0.6 mL/min
- UV-detection at 227 nm
- Column:
- Eurospher II 100-5 C18p by [http://www.knauer.net/ Knauer]
- Dimensions: 150 x 4.6 mm with precolumn
- Particle size: 5 µm
- Pore size: 100 Å
- Material: silica gel
