Team:Caltech/Recipes
From 2011.igem.org
(Difference between revisions)
Line 48: | Line 48: | ||
* 6.25 μl of Phusion polymerase | * 6.25 μl of Phusion polymerase | ||
* 216.75 μl of Nuclease-free water | * 216.75 μl of Nuclease-free water | ||
- | * (375 μl total) | + | * (375 μl total) <br/><br/> |
- | <br/> | + | |
+ | <p>'''IPTG stock'''</p> | ||
+ | For 1000x stock (.1M) | ||
+ | *.0238 g IPTG | ||
+ | *1 mL sterile water<br/><br/> | ||
<p>'''p450 degradation testing solution'''</p> | <p>'''p450 degradation testing solution'''</p> |
Revision as of 23:58, 28 August 2011
Project |
Back to Timeline . Back to Methods 50% glycerol Stock:
Agar/LB plate (Autoclaved):
Ampicillin Stock
Chloramphenicol Stock
Enrichment Minimal Media
Gibson Mix (1.33x) For 25 aliquots of 15 μl each:
IPTG stock For 1000x stock (.1M)
p450 degradation testing solution For a 200uL reaction:
Phusion PCR For a 50uL reaction:
SOC Media for 250 ml (adapted from [http://openwetware.org/wiki/SOC OpenWetWare])
Taq PCR (for 16s insert) For a 25uL reaction:
X-gal stock (50x) For 20mg/mL, total 0.5mL volume:
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