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Team:NTNU Trondheim/Stress Sensor
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The stress sensor is our main construct. It is made from the following biobricks: | The stress sensor is our main construct. It is made from the following biobricks: | ||
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| + | [[File:construct_map_1807.jpg|thumb|Plasmid map of the complete construct. Promoters are shown in blue, and genes as inside arrows. Only single restriction sites are shown.]] | ||
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Revision as of 11:50, 26 August 2011
Stress sensor (rrnB+LacI+pLac+mCherry)
The stress sensor is our main construct. It is made from the following biobricks:
| Biobrick | Part number |
|---|---|
| LacI with RBS | BBa_J24679 |
| Terminator | BBa_B0015 |
| pLac | BBa_R0011 |
| mCherry with RBS and terminator | BBa_J06702 |
The promotor is a variant of BBa_K112118, that we have made ourselves using PCR. The stress sensor has a total length of 2653 bp, and is located inside the plasmid pSB1A2, carrying ampicillin resistance. We have put the stress sensor through several tests (see characterization)
