Team:St Andrews

From 2011.igem.org

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<p class="textpart"> Antimicrobial Peptides have been an area of great interest for many years.  They form part of an evolutionarily conserved innate immune response.</p>
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<p class="textpart"> For the 2011 St Andrews iGEM Team project, we are creating an intracellular Escherichia coli “kill switch” that functions differently from any found in nature.  This “kill switch” is a tool, one which we believe will have application within many areas of biology.</p>
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<p class="textpart"> For the 2011 St Andrews iGEM project we are creating a ‘kill switch’ where we will be inserting a gene (protegrin-1) coding for Anti-Microbial Peptides (AMP’s) into E. Coli.  AMP’s cause the death of bacterial cells by displacement of lipids and alteration of the membrane structure.  The idea is to then use different promoters to switch on the expression of these proteins in certain conditions (we envisage that this would have many practical applications, some of which we hope to maybe explore later on).</p>
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<p class="textpart"> Our kill switch is designed by inserting an antimicrobial peptide (AMP) gene into E. coli.  The AMP in question is protegrin-1, an 18 amino acid residue first identified in porcine leukocytes.  Protegrin-1 has high microbicidal activity against E. coli (gram-negative), N. gonorrheae (gram-positive), and HIV-1 (lipid-coated virus), amongst several other bacterial and virion species.  It is an evolutionarily conserved peptide found within the innate immune system of </p>
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Revision as of 10:32, 26 August 2011

Description
Project Diary
Safety Form
The Kill Switch
Modelling
Parts Submitted
Drug Delivery
Biosafety
Conjugation
"Is iGEM Fair?"
Panel Debate
Reflective Essays
The Team
Sponsors
Collaboration

Project Description

For the 2011 St Andrews iGEM Team project, we are creating an intracellular Escherichia coli “kill switch” that functions differently from any found in nature. This “kill switch” is a tool, one which we believe will have application within many areas of biology.

Our kill switch is designed by inserting an antimicrobial peptide (AMP) gene into E. coli. The AMP in question is protegrin-1, an 18 amino acid residue first identified in porcine leukocytes. Protegrin-1 has high microbicidal activity against E. coli (gram-negative), N. gonorrheae (gram-positive), and HIV-1 (lipid-coated virus), amongst several other bacterial and virion species. It is an evolutionarily conserved peptide found within the innate immune system of

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