"
Team:NTNU Trondheim/Stress Sensor
From 2011.igem.org
(Difference between revisions)
m (→Stress sensor (rrnB+LacI+pLac+mCherry)) |
|||
| Line 29: | Line 29: | ||
The stress sensor has a total length of 2653 bp, and is located inside the plasmid pSB1A2, carrying ampicillin resistance. | The stress sensor has a total length of 2653 bp, and is located inside the plasmid pSB1A2, carrying ampicillin resistance. | ||
We have put the stress sensor through several tests (see [https://2011.igem.org/Team:NTNU_Trondheim/stress-sensor characterization]) | We have put the stress sensor through several tests (see [https://2011.igem.org/Team:NTNU_Trondheim/stress-sensor characterization]) | ||
| + | |||
| + | |||
| + | {{:Team:NTNU_Trondheim/NTNU_footer}} | ||
Revision as of 08:23, 26 August 2011
Stress sensor (rrnB+LacI+pLac+mCherry)
The stress sensor is our main construct. It is made from the following biobricks:
| Biobrick | Part number |
|---|---|
| LacI with RBS | BBa_J24679 |
| Terminator | BBa_B0015 |
| pLac | BBa_R0011 |
| mCherry with RBS and terminator | BBa_J06702 |
The promotor is a variant of BBa_K112118, that we have made ourselves using PCR. The stress sensor has a total length of 2653 bp, and is located inside the plasmid pSB1A2, carrying ampicillin resistance. We have put the stress sensor through several tests (see characterization)
