Team:Caltech/Week 11
From 2011.igem.org
(Difference between revisions)
Line 15: | Line 15: | ||
* 16 LB-chlor plates | * 16 LB-chlor plates | ||
* 13 LB plates | * 13 LB plates | ||
+ | ==August 24== | ||
+ | ==August 25== | ||
+ | ===Results=== | ||
+ | <table border="1"> | ||
+ | <tr> | ||
+ | <th>Name</th> | ||
+ | <th>Number of colonies</th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>p450 biobricked in pSB1C3</td> | ||
+ | <td>~3400</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>DDT gene biobrick now with stop codons in pSB1C3</td> | ||
+ | <td>~6400</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>pSB1C3 ligation negative control</td> | ||
+ | <td>0</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>DDT gene inserted into pET11-a</td> | ||
+ | <td>~6400</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>pET11-a ligation negative control</td> | ||
+ | <td>27</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>pUC 19 in DH5a electrocompetent cells</td> | ||
+ | <td>976</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>DDT gene PIPE</td> | ||
+ | <td>0</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>VPIPE negative control</td> | ||
+ | <td>0</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <tr> | ||
+ | <td>pNT002 + in commercial XL-10 gold</td> | ||
+ | <td>54</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>pNT002 - in commercial XL-10 gold</td> | ||
+ | <td>28</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | </table> | ||
}} | }} |
Revision as of 22:25, 25 August 2011
Project |
August 22Send BPA and degraded products to HPLC for analysis ResultsPlated 16s and WT-F87A cultures show many colonies August 23Make experimental MG-1655 strain electrocompetent
August 24August 25Results
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