Team:Caltech/Week 11

From 2011.igem.org

(Difference between revisions)
Line 4: Line 4:
==August 22==
==August 22==
Send BPA and degraded products to HPLC for analysis<br/>
Send BPA and degraded products to HPLC for analysis<br/>
 +
Send 16s for sequencing using FWD and REV. <br/>
===Results===
===Results===
Plated 16s and WT-F87A cultures show many colonies<br/>
Plated 16s and WT-F87A cultures show many colonies<br/>
Line 10: Line 11:
==August 23==
==August 23==
Make experimental MG-1655 strain electrocompetent<br/>
Make experimental MG-1655 strain electrocompetent<br/>
 +
Made 53 plates today!<br/>
 +
* 24 LB-amp plates
 +
* 16 LB-chlor plates
 +
* 13 LB plates
}}
}}

Revision as of 19:43, 25 August 2011


Caltech iGEM 2011



Home

Project

Data

Parts

Team

Notebook

Biosafety

Human Impact

References

Support

August 22

Send BPA and degraded products to HPLC for analysis
Send 16s for sequencing using FWD and REV.

Results

Plated 16s and WT-F87A cultures show many colonies
Mix 10% glycerol solution and place in 4&degC fridge for tomorrow's electrocompetence method
Overnight shake culture of MG-1655 experimental E. coli strain

August 23

Make experimental MG-1655 strain electrocompetent
Made 53 plates today!

  • 24 LB-amp plates
  • 16 LB-chlor plates
  • 13 LB plates


Retrieved from "http://2011.igem.org/Team:Caltech/Week_11"