Team:SouthBend-Mishawaka-HS/Notebook

From 2011.igem.org

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(10)Plated 100 microliters and 25 microliters of transformed cells on LB-AMP (5-19-11 "crew")
(10)Plated 100 microliters and 25 microliters of transformed cells on LB-AMP (5-19-11 "crew")
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==May 18==
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We found no growth on the plates. We are going to re-transform them next week, this time using electricity instead of heat-shock.
==May 24==
==May 24==
(1)Obtained 40 microliters of electrocompetent TOP-10 <i>E. coli</i> cells.<br>
(1)Obtained 40 microliters of electrocompetent TOP-10 <i>E. coli</i> cells.<br>
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(4)Recovered with 900 microliters of SOB to recover cells<br>
(4)Recovered with 900 microliters of SOB to recover cells<br>
(5)Incubated for 1hr. Then plated the newly transformed cells.<br>
(5)Incubated for 1hr. Then plated the newly transformed cells.<br>
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
 
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!align="center"|[[Team:SouthBend-Mishawaka-HS|Home]]
 
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!align="center"|[[Team:SouthBend-Mishawaka-HS/Team|Team]]
 
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=SouthBend-Mishawaka-HS Official Team Profile]
 
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!align="center"|[[Team:SouthBend-Mishawaka-HS/Project|Project]]
 
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!align="center"|[[Team:SouthBend-Mishawaka-HS/Parts|Parts Submitted to the Registry]]
 
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!align="center"|[[Team:SouthBend-Mishawaka-HS/Modeling|Modeling]]
 
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!align="center"|[[Team:SouthBend-Mishawaka-HS/Notebook|Notebook]]
 
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!align="center"|[[Team:SouthBend-Mishawaka-HS/Safety|Safety]]
 
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!align="center"|[[Team:SouthBend-Mishawaka-HS/Attributions|Attributions]]
 
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Revision as of 20:36, 26 May 2011


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Home The Team The Project Notebook Safety


Contents

May

May19

(1)Poured 12 LB AMP plates:(2mg of Ampicillin to 250ml of Agar)
(2)Obtained a vial of 2 microliters of TOP-10 electrocompetant E. coli cells (44-0003/793762)
(3)Added 50 microliters of pure water from the Gemini machine
(4)Added 10 ul of water to well 6E of plate 2, which contained part F2620.
(5)Added 2 ul of the diluted DNA in 50 ul of transformation solution (50mm CaCl2, pH 6.1 BIO-RAD Transformation Solution control # 31000008916 recieved 1-17-11 GT).
(6)Incubate on ice for 5 minutes
(7)Heat shocked at 40 degrees Celcius for 50 seconds
(8)Incubated at 4 degrees Celcius for 5 minutes
(9)Added 1mL of LB (5-17-11 DG)
(10)Plated 100 microliters and 25 microliters of transformed cells on LB-AMP (5-19-11 "crew")

May 18

We found no growth on the plates. We are going to re-transform them next week, this time using electricity instead of heat-shock.

May 24

(1)Obtained 40 microliters of electrocompetent TOP-10 E. coli cells.
(2)Added 2 microliters of F2620.
(3)Zapped with 1.8kV for 5.8ms.
(4)Recovered with 900 microliters of SOB to recover cells
(5)Incubated for 1hr. Then plated the newly transformed cells.

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