Team:Freiburg/Notebook/19 August

From 2011.igem.org

(Difference between revisions)
(→Meeting)
(→Meeting)
Line 9: Line 9:
already done:
already done:
-
 
+
*Ccas some colonies (check if positive)
 +
*CcaR still doesn't work
To-do:
To-do:
 +
*cph8 still won't work, we should ask to get the original sequence
===<span style="color:blue;">blue light receptor</span>===
===<span style="color:blue;">blue light receptor</span>===
already done:
already done:
-
 
+
*receptor and NOT-Gate assembly
To-do:
To-do:
 +
*add Promotor-RBS and terminator
===<span style="color:red;">red light receptor</span>===
===<span style="color:red;">red light receptor</span>===
already done:
already done:
-
 
+
*Promotor-RBS-ho1-terminator ready
 +
*Promotor-RBS-pcyA-terminator ready
To-do:
To-do:
-
 
+
*receptor still doesn't work (not possible to amplify via a PCR)
===<span style="color:orange;">Lysis cassette</span>===
===<span style="color:orange;">Lysis cassette</span>===
already done:
already done:
-
 
+
*quick change with the 11 bases didn't work
 +
*did a 3A assembly with the single parts
To-do:
To-do:
Line 38: Line 43:
already done:
already done:
 +
*finally the genes arrived
 +
*GST from the bioss toolkit can't be amplified from the supported vector, Rüdiger should do a positive control next time, if it still doesn't work he should get new primer pairs
 +
To-do:
 +
*PBD + inducible promotor
-
 
+
===other stuff===
-
To-do:
+
*Hauke Busch recommended CellDesigner to Rüdiger to do the modelling, Rüdiger will download it and try to install it
 +
*modelling should be done for the precipitator, escpecially the Ni-Histidin-binding and for the plastic binding domain, maybe also the GST-Tag affinity
 +
*Tobi will organize moving the stuff out of the lab after the wiki freeze
 +
*Sandra will ask for the appointment to talk at the MPI
<br/>
<br/>
<br/>
<br/>

Revision as of 18:57, 24 August 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

Meeting

attendants: Jakob, Julia, Manuel, Rüdiger, Sandra, Sophie, Theo
Time: 9:00 - 10:00

green light receptor

already done:

  • Ccas some colonies (check if positive)
  • CcaR still doesn't work

To-do:

  • cph8 still won't work, we should ask to get the original sequence

blue light receptor

already done:

  • receptor and NOT-Gate assembly

To-do:

  • add Promotor-RBS and terminator

red light receptor

already done:

  • Promotor-RBS-ho1-terminator ready
  • Promotor-RBS-pcyA-terminator ready

To-do:

  • receptor still doesn't work (not possible to amplify via a PCR)

Lysis cassette

already done:

  • quick change with the 11 bases didn't work
  • did a 3A assembly with the single parts

To-do:


Precipitator

already done:

  • finally the genes arrived
  • GST from the bioss toolkit can't be amplified from the supported vector, Rüdiger should do a positive control next time, if it still doesn't work he should get new primer pairs

To-do:

  • PBD + inducible promotor

other stuff

  • Hauke Busch recommended CellDesigner to Rüdiger to do the modelling, Rüdiger will download it and try to install it
  • modelling should be done for the precipitator, escpecially the Ni-Histidin-binding and for the plastic binding domain, maybe also the GST-Tag affinity
  • Tobi will organize moving the stuff out of the lab after the wiki freeze
  • Sandra will ask for the appointment to talk at the MPI



green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME


blue light receptor

Sandra, Sophie: primer design for NOT-gate to get NEH I restriction sites inside instead of Spe I. We can not cut with Spe because our trp-promoter contains Spe restriction sites.

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

NAME OF YOUR EXPERIMENT

Investigators: NAME

                       

Retrieved from "http://2011.igem.org/Team:Freiburg/Notebook/19_August"