Team:Freiburg/Notebook/24 August

From 2011.igem.org

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Repeat of the transformation from yesterday with the Lovtap-NotGate-pSB1A3 vector.
Repeat of the transformation from yesterday with the Lovtap-NotGate-pSB1A3 vector.
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'''PCR'''
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===PCR===

Revision as of 11:54, 24 August 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

Digest

Investigators: Jakob

  • 10µl BSA
  • 10µl NEB
  • 2µl DpnI
  • 38µl DNA

Digest for 2h at 37°C

blue light receptor

Transformation

Investigators: Sandra

Repeat of the transformation from yesterday with the Lovtap-NotGate-pSB1A3 vector.

PCR

Name: Sophie


Date: 24.08.11
Continue from Experiment: primer design (Date) 19.08.11

(Name) Sandra

Project Name: Blue light

PCR-Mixture for one Reaction:

For a 50 µl reaction use


32,5µl H20 Name
10µl 5x Phusion Buffer of Primer
2.5µl Primer fw P24
2.5µl Primer dw P79
1µl dNTPs of Template DNA
1µl DNA-Template M45
0.5 µl Phusion (add in the end)

What program do you use?

20 cycles with an annealing temperature of 65/68/71 ºC (gradient-PCR) then 10 cycles with an annealing temperature of 65/70/75 ºC


To confirm the PCR-Product has the correct size, load 2 µl of the sample onto an agarose-gel.


How did you label the PCR-Product, where is it stored and what do you do next?

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

NAME OF YOUR EXPERIMENT

Investigators: NAME

                       

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