Team:XMU-China/Project

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A bacterial ‘population-control’ device
A bacterial ‘population-control’ device
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== Project Details==
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== Project Abstract==
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We have developed a series of devices which program a bacteria population to maintain at different cell densities. We have designed and characterized the genetic circuit to establish a bacterial ‘population-control’ device in E. coli based on the well-known quorum-sensing system from Vibrio fischeri, which autonomously regulates the density of an E. coli population. The cell density however is influenced by the expression levels of a killer gene (ccdB) in our device. As such, we have regulated the expression levels of the killer gene (ccdB) by site-directed mutagenesis of a luxR promoter (PluxR) and error-prone PCR of gene luxR, and finally we have built a database for a series of mutation sites corresponding to different cell densities. An artificial neural network has then been built to model and predict the cell density of an E. coli population. This work can serve as a foundation for future advances involving fermentation industry and food industry.
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=== Backgrounds ===
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=== Part 2 ===
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=== Project Detail===
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Although this area is pretty new to us, we have successfully built up the framework and logic formula for the circuit. Our genetic circuit is mainly made up of a group of promoters, inducers, repressors and report sequences. They are designed to function as a “monitor”, a device which can detect the existence of some substances and give out visualized signals by the cells. However, to finally make the circuit work as we supposed, there is still a long way to go. As a lot of promoters are involved, the circuit gets more convoluted than we thought. We have to find out appropriate substances and promoters which can interact with each other as well as meet the logic formula. We are now searching for different kinds of promoters which are available in the standard biobrick list and designing combinatorial promoters to fit the circuit. Meanwhile, there are some other factors like the time-delay between two genetic parts and the stability of the device which need to be considered. We are not clear whether we can finally overcome all these difficulties, but we are clear that this project is worth doing and everything worth doing is worth doing well. We will keep updating our experiment data and more details.
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=== The Experiments ===
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=== Part 3 ===
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== Results ==
== Results ==

Revision as of 06:22, 24 August 2011


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Contents

Project Name

A bacterial ‘population-control’ device

Project Abstract

We have developed a series of devices which program a bacteria population to maintain at different cell densities. We have designed and characterized the genetic circuit to establish a bacterial ‘population-control’ device in E. coli based on the well-known quorum-sensing system from Vibrio fischeri, which autonomously regulates the density of an E. coli population. The cell density however is influenced by the expression levels of a killer gene (ccdB) in our device. As such, we have regulated the expression levels of the killer gene (ccdB) by site-directed mutagenesis of a luxR promoter (PluxR) and error-prone PCR of gene luxR, and finally we have built a database for a series of mutation sites corresponding to different cell densities. An artificial neural network has then been built to model and predict the cell density of an E. coli population. This work can serve as a foundation for future advances involving fermentation industry and food industry.


Backgrounds

Project Detail

Results

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