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Team:Lyon-INSA-ENS/Realisation/Week10
From 2011.igem.org
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| + | Both NM522 and NM522/pIG24 grew on LB+Amp ( liquid and solid ) : antibiotic concentration in liquid was not sufficient, a more concentrated solution of Amp (10mg/mL which corresponds to 100X) is made and sterilized by filtration.<br/> | ||
| + | Creation of our own LB+Amp plates by plating 200µL of Amp on a LB plate.<br/> | ||
| + | Plating of several AmpS strains on the previous plates : PHL818, MC4100, NM522+pIG4, NM522 from the collection, NM522 from the previous LB+Amp plate, a new NM522 found in another lab.<br/><br/> | ||
| + | |||
| + | Plating of 10µL of the new NM522 on LB <br/><br/> | ||
| + | |||
| + | Dissolution of 0.08g of Spectinomycin ( C14H24N2O7 + 2 HCl + 5 H20 ) into 5mL water and filtration to obtain a solution at 10mg/mL.<br/><br/> | ||
| + | |||
| + | QIAGen extraction of NM522 and NM522+pIG4 and electrophoresis : no plasmid is in the strains. | ||
</p> | </p> | ||
Revision as of 14:15, 23 August 2011
Week 10
From Monday the 22th of August to Friday the 26th of August 2011
Monday
Both NM522 and NM522/pIG24 grew on LB+Amp ( liquid and solid ) : antibiotic concentration in liquid was not sufficient, a more concentrated solution of Amp (10mg/mL which corresponds to 100X) is made and sterilized by filtration.
Creation of our own LB+Amp plates by plating 200µL of Amp on a LB plate.
Plating of several AmpS strains on the previous plates : PHL818, MC4100, NM522+pIG4, NM522 from the collection, NM522 from the previous LB+Amp plate, a new NM522 found in another lab.
Plating of 10µL of the new NM522 on LB
Dissolution of 0.08g of Spectinomycin ( C14H24N2O7 + 2 HCl + 5 H20 ) into 5mL water and filtration to obtain a solution at 10mg/mL.
QIAGen extraction of NM522 and NM522+pIG4 and electrophoresis : no plasmid is in the strains.
Tuesday
Wednesday
Thursday
Friday
