Team:Freiburg/Notebook/22 August

From 2011.igem.org

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'''Investigators: Sophie'''
'''Investigators: Sophie'''
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new 3A assembly with Sandra's NOT-gate-PCR-product, our LOV-Tap-pcr-product (both mutated with NEH1 restriction sites) and an amp-vector
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new 3A assembly with Sandra's NOT-gate-PCR-product, our LOV-Tap-pcr-product (both mutated with NEH1 restriction sites) and an amp-vector <br/>
==<span style="color:red;">red light receptor</span>==
==<span style="color:red;">red light receptor</span>==

Revision as of 12:56, 23 August 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME


blue light receptor

Sequencing

Investigators: Sandra

The sequencing showed that the 3A-assembly did not work. We will send the LovTap PCR product to get sequenced. May be the PCR did not work.

PCR

Investigators: Sandra PCR of Not-Gate.

P 74:

  • actgcagcggccgctgctagca

P 24:

  • tatgaattcgcggccgcttctag


PCR product was loaded onto a gel. Expected lane: 900bp

Cloning

Investigators: Sophie new 3A assembly with Sandra's NOT-gate-PCR-product, our LOV-Tap-pcr-product (both mutated with NEH1 restriction sites) and an amp-vector

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

Transformation

Investigators: Rüdiger, Sandra

Transformation of 61,62 and 63.
Vector:pSB1C3

Incubation over night.


Cloning for GFP-pbd with IPTG-inucible promoter

Investigator: Sophie
as cloning from 18.08.11 did not work I try it again with longer digestion times.

                       

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