Team:NTNU Trondheim/LacI+pLac+mCherry

From 2011.igem.org

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== Stress sensor precursor (LacI+pLac+mCherry) ==
== Stress sensor precursor (LacI+pLac+mCherry) ==
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[[File:LacI+pLac+mCherry_rosa.png|780px]]
[[File:LacI+pLac+mCherry_rosa.png|780px]]
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This is our system without the rrnB P1-promotor.
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This is our system without the rrnB P1-promotor. Because this construct lacks a downregulated promotor in front of it, LacI will not be syntethized, and in response, pLac will not be downregulated of LacI. Since pLac is not downregulated, mCherry is going to be present whether the cells are stressed or not.
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Our experiments indicate that the stress sensor precursor is put together the right way.

Revision as of 19:57, 22 August 2011



Stress sensor precursor (LacI+pLac+mCherry)

LacI+pLac+mCherry rosa.png

This is our system without the rrnB P1-promotor. Because this construct lacks a downregulated promotor in front of it, LacI will not be syntethized, and in response, pLac will not be downregulated of LacI. Since pLac is not downregulated, mCherry is going to be present whether the cells are stressed or not. Our experiments indicate that the stress sensor precursor is put together the right way.