Team:Freiburg/Notebook/22 August

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===Cloning for GFP-pbd with IPTG-inucible promoter===
===Cloning for GFP-pbd with IPTG-inucible promoter===
'''Investigator: Sophie'''
'''Investigator: Sophie'''
 +
<br/>
as cloning from 18.08.11 did not work I try it again with longer digestion times.
as cloning from 18.08.11 did not work I try it again with longer digestion times.
Incubation over night.
Incubation over night.
{{:Team:Freiburg/Templates/footer}}
{{:Team:Freiburg/Templates/footer}}

Revision as of 14:43, 22 August 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME


blue light receptor

Sequencing

Investigators: Sandra

The sequencing showed that the 3A-assembly did not work. We will send the LovTap PCR product to get sequenced. May be the PCR did not work.

PCR

Investigators: Sandra PCR of Not-Gate.

P 74:

  • actgcagcggccgctgctagca

P 24:

  • tatgaattcgcggccgcttctag


PCR product was loaded onto a gel. Expected lane: 900bp

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

Transformation

Investigators: Rüdiger, Sandra

Transformation of 61,62 and 63.
Vector:pSB1C3


Cloning for GFP-pbd with IPTG-inucible promoter

Investigator: Sophie
as cloning from 18.08.11 did not work I try it again with longer digestion times.

Incubation over night.

                       

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