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Team:Cambridge/Experiments/Assembly of Reflectin Constructs
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===PCR=== | ===PCR=== | ||
In the first round of PCR, we amplified fragments required for the assembly of GA1, GA2, GA3 and GA4 constructs. | In the first round of PCR, we amplified fragments required for the assembly of GA1, GA2, GA3 and GA4 constructs. | ||
| + | *We performed PCR using [[Team:Cambridge/Protocols/PCR#Reagents_used_in_PCR_reaction | Phusion Hot Start DNA Polymerase]] in 20 μl reaction volume. | ||
===Gibson Assembly=== | ===Gibson Assembly=== | ||
Revision as of 11:26, 19 August 2011
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Contents |
Construct Design
Primer Design
Assembly: first attempt
PCR
In the first round of PCR, we amplified fragments required for the assembly of GA1, GA2, GA3 and GA4 constructs.
- We performed PCR using Phusion Hot Start DNA Polymerase in 20 μl reaction volume.
Gibson Assembly
Transformation
Results
Diagnostics
Assembly: second attempt
PCR
Gibson Assembly
Transformation
Results
What next?
