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Team:Caltech/Week 10
From 2011.igem.org
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GCMS results show no correct mass spectrometry peaks. | GCMS results show no correct mass spectrometry peaks. | ||
==August 16== | ==August 16== | ||
| - | Electrospray bisphenol A dissolved in methanol<br/> | + | Electrospray bisphenol A dissolved in methanol<br/> |
| + | Attempt to standard assemble constitutively expressed LacZ and transform into XL10 gold. However, there was no good way to select out the lacZ insert (I732019) from the backbone it was cut out of, as they are the same length.<br/> | ||
| + | |||
===Results=== | ===Results=== | ||
Electrospray results show correct mass spectrometry peaks<br/> | Electrospray results show correct mass spectrometry peaks<br/> | ||
| + | ==August 17== | ||
| + | Borrowed HindIII from Nate to be able to gel extract lacZ from the now smaller backbone pieces<br/> | ||
| + | Transformed psB1C3 into top10 cells<br/> | ||
| + | ===Results=== | ||
| + | The lacZ plated on LB-amp X-gal had about 520 colonies. The negative control had around 700 colonies. None of the colonies were blue, likely indicating that there was no functioning lacZ. Some were sent off for sequencing.<br/> | ||
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Revision as of 17:30, 18 August 2011
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Project |
August 15CPCR of Gibson pNT002 and pNT003 ResultsCPCR gels showed 200 bp bands, indicating self-ligation of all colonies picked. August 16Electrospray bisphenol A dissolved in methanol ResultsElectrospray results show correct mass spectrometry peaks August 17Borrowed HindIII from Nate to be able to gel extract lacZ from the now smaller backbone pieces ResultsThe lacZ plated on LB-amp X-gal had about 520 colonies. The negative control had around 700 colonies. None of the colonies were blue, likely indicating that there was no functioning lacZ. Some were sent off for sequencing.
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