Team:Osaka/week3
From 2011.igem.org
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!ID!!Part Name!!Resistance!!Description | !ID!!Part Name!!Resistance!!Description | ||
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- | |1- | + | |1-6I||<bbpart>BBa_R0040</bbpart>||A|| TetR repressible promoter |
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# Miniprep of yesterday's culture | # Miniprep of yesterday's culture |
Revision as of 13:33, 18 August 2011
Contents |
August 16(Tue)
- Gel electrophoresis of 01,02,03,04,05,06
- Transfer to LB culture medium:01,02,03,04,05,06,1-23L
- Ligation
- 8/5 3. the same way
- Transformation of Ligation products
August 17(Wed)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Restriction digests of 03,05
- Gel electrophoresis of digests
- Ligation
- 07(C):05(upstream)+2-16N(downstream)+1-3A(vector)
- Transfer to LB culture midium:01,02,04,05,06
August 18(Thu)
- Transfer to LB culture midium:1-3A,1-5A,1-1G,03,1-23L
- Transformation of 07 and Resistry parts (See Table 3)
ID | Part Name | Resistance | Description |
---|---|---|---|
1-6I | <bbpart>BBa_R0040</bbpart> | A | TetR repressible promoter |
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests