Team:Osaka/week3
From 2011.igem.org
(Difference between revisions)
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# Ligation | # Ligation | ||
#* 07(C):05(upstream)+2-16N(downstream)+1-3A(vector) | #* 07(C):05(upstream)+2-16N(downstream)+1-3A(vector) | ||
- | # Transfer to LB culture midium:01,02 | + | # Transfer to LB culture midium:01,02,04,05,06 |
+ | |||
+ | |||
+ | ==August 18(Thu)== | ||
+ | # Transfer to LB culture midium:1-3A,1-5A,1-1G,03,1-23L | ||
+ | # Transformation of 07 and Resistry parts (See Table 3) | ||
+ | {|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5" | ||
+ | |+Table2 | ||
+ | !ID!!Part Name!!Resistance!!Description | ||
+ | |- | ||
+ | |1-23L||<bbpart>BBa_R0040</bbpart>||A|| TetR repressible promoter | ||
+ | |} | ||
+ | # Miniprep of yesterday's culture | ||
+ | # Restriction digests of minipreppped parts | ||
+ | # Gel electrophoresis of digests | ||
+ | |||
+ | ==August 19(Fri)== | ||
+ | |||
+ | |||
+ | |||
+ | ==August 20(Sat)== |
Revision as of 13:32, 18 August 2011
Contents |
August 16(Tue)
- Gel electrophoresis of 01,02,03,04,05,06
- Transfer to LB culture medium:01,02,03,04,05,06,1-23L
- Ligation
- 8/5 3. the same way
- Transformation of Ligation products
August 17(Wed)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Restriction digests of 03,05
- Gel electrophoresis of digests
- Ligation
- 07(C):05(upstream)+2-16N(downstream)+1-3A(vector)
- Transfer to LB culture midium:01,02,04,05,06
August 18(Thu)
- Transfer to LB culture midium:1-3A,1-5A,1-1G,03,1-23L
- Transformation of 07 and Resistry parts (See Table 3)
ID | Part Name | Resistance | Description |
---|---|---|---|
1-23L | <bbpart>BBa_R0040</bbpart> | A | TetR repressible promoter |
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests