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Team:UT Dallas/Protocols
From 2011.igem.org
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<h2><span></span>Ligation Protocol</h2> | <h2><span></span>Ligation Protocol</h2> | ||
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| - | <p><li type = "disk"><li type = "disk"><li type = "disk">< | + | <p><li type = "disk">Determine insert to vector ratios<li type = "disk">Calculate the amount of insert needed if 50ng of vector is used (can use different amount of vector)<li type = "disk">In a PCR tube add the following:<blockquote><li type = "circle">50ng of vector<li type = "circle">Amount of insert based on ratios (calculated in second step)<li type = "circle">2uL of buffer<li type = "circle">2uL of DNA ligase<li type = "circle">Amount of water to bring total volume to 20uL</blockquote><li type = "disk">Incubate overnight at 14oC</p></li><p>Note: We used T4 DNA ligase and buffer from NEB</p> |
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<h2><span></span>Gel Purification Protocol (QIAquick Gel Extraction Kit)</h2> | <h2><span></span>Gel Purification Protocol (QIAquick Gel Extraction Kit)</h2> | ||
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Revision as of 16:01, 17 August 2011
