Team:UT Dallas/Protocols

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       <h1><span>UTDallas</span> iGem<small></small></h1>
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           <h3><span>Protocols</span></h3>
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           <h2><span> <b>Protocols</b></span></h2>
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  <blockquote><h2><span></span>Ligation Protocol</h2>
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  <h3><span></span>Gel Purification Protocol (from QIAquick Gel Extraction Kit)</h3>
 
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  <h3><span></span>Preparing LB + Appropriate Antibiotic Protocol</h3>
 
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  <h3><span></span>Preparing Agar Plates Protocol (Makes 12 (15mm) Plates)</h3>
 
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  <h3><span></span>Miniprep Protocol (from QIAprep Spin Miniprep Kit)</h3>
 
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  <h3><span></span>Preparing Glycerol Stock Protocol</h3>
 
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<h3><span></span>Transformation Protocol</h3>
 
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          <p> <font size="5" face="arial" color = "#10314B">Ligation Protocol</font></p>
 
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          <li type = "square">Determine insert to vector ratios<li type = "square">Calculate the amount of insert needed if 50ng of vector is used (can use different amount of vector)<li type = "square">In a PCR tube add the following:<blockquote><li type = "circle">50ng of vector<li type = "circle">Amount of insert based on ratios (calculated in second step)<li type = "circle">2uL of buffer<li type = "circle">2uL of DNA ligase<li type = "circle">Amount of water to bring total volume to 20uL</blockquote><li type = "square">Incubate overnight at 14oC<p>Note: We used T4 DNA ligase and buffer from NEB
 
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             <li><a href="https://2011.igem.org/Team:UT_Dallas/Project"><font size="3" face="verdana">Project</a></font></li>
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             <li><a href="https://2011.igem.org/Team:UT_Dallas/Notebook"><font size="3" face="verdana">Notebook</a></font></li>
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       <h2><span><font size="5" face="arial">UT Dallas</span></font></h2>
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       </font></p><p><a href="https://2011.igem.org/Team:UT_Dallas/UTDallas"><font size="3" face="verdana">Click here to learn more&nbsp; about UTD</a></font></p>
       </font></p><p><a href="https://2011.igem.org/Team:UT_Dallas/UTDallas"><font size="3" face="verdana">Click here to learn more&nbsp; about UTD</a></font></p>
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Revision as of 15:43, 17 August 2011

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