Team:Freiburg/Notebook/10 August

From 2011.igem.org

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==<span style="color:green;">green light receptor</span>==
==<span style="color:green;">green light receptor</span>==
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===NAME OF YOUR EXPERIMENT===
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===Results from the Transformation===
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'''Investigators:NAME'''
+
'''Investigators: Jakob'''
 +
Results are again negativ...
 +
*To-do: Tomorrow we will have a call with the iGEM-Team from Uppsala Sweden, maybe they can give us some favour.
==<span style="color:blue;">blue light receptor</span>==
==<span style="color:blue;">blue light receptor</span>==

Revision as of 11:01, 17 August 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

Results from the Transformation

Investigators: Jakob

Results are again negativ...

  • To-do: Tomorrow we will have a call with the iGEM-Team from Uppsala Sweden, maybe they can give us some favour.

blue light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



red light receptor

3A-assembly

Investigators: Sandra

Digestion

  • PR1 (pSB1C3)
  • PR2 (pSB1C3)
  • pcyA (pSB1T3)
  1. pcyATd (pSB1T3)
  2. pcyATb (pSB1T3)
  • L23 (pSB1T3)
  • vector (pSB1K3)


Amount of DNA and H20:

Sample 500(ng)/DNA concentration (ng/μl) H20 μl
pcyATd 2.8 35.2
pcyaTb 2.9 35.1
PR2 7.2 30.8
PR1 6.1 31.9
L23 4.8 33.2
pSB1K3 20 18


Enzymes necessary for digestion:

PR1/PR2 pcyA/L23 vector
enzyme 1 EcoRI XbaI EcoRI
enzyme 2 SpeI PstI PstI


-incubated for 1 hours at 37°C.
-heated for 20 minutes at 80°C


Ligation

Ligation of:

PcayTd PcyATb L23 (ho1)
PR1 1 pTd 1 pTb 1 L23
PR2 2 pTd 2 pTb 2 L23

Named the samples, see above in table.

Transformation

Transformation of ligated part:

  • 1pTd (PR1-pcyATd)
  • 2pTd (PR2-pcyATd)
  • 1pTb (PR1-pcyATb)
  • 2pTb (PR2-pcyATb)
  • 1L23 (PR1-Ho1T)
  • 2L23 (PR2-Ho1T)


plated out on plates with kanamycin resistance.

Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

NAME OF YOUR EXPERIMENT

Investigators: NAME

                       

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