Team:UC Davis/Notebook/Week 10

From 2011.igem.org

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Week 10 to be updated!
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--Monday 8/15/11--
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We got our sequences from last week in and checked them to make sure they looked good. Not all of the parts were correct, but we are about a day or two from being done with construction!
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Here is a table of which parts are completed and which parts we need to complete in order to finish construction.
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<table>
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<tr><td></td><td><b>LacI</b></td><td><b>c1</b></td><td><b>TetR</b></td></tr>
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<tr><td>Repressor Screening</td><td>*R0010+E0240+I13453+B0034</td><td>R0051+E0240+I13453+B0034 </td><td>*R0040+E0240+I13453+B0034</td></tr>
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<tr><td>Promoter Screening</td><td>E0240+I13453+B0034+C0012</td><td>E0240+I13453+B0034+C0051</td><td>E0240+I13453+B0034+C0040</td></tr>
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<tr><td>Wild Type Controls</td><td>*R0010+E0240+I13453+B0034+C0012 </td><td>R0051+E0240+I13453+B0034+C0051</td><td>*R0040E0240+I13453+B0034+C0040</td></tr>
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</table>
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{| border="1"
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|''' '' || '''LacI''' || '''c1'''||'''TetR'''
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|-
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|'''Repressor Screening''' || R0010+E0240+I13453+B0034 || R0051+E0240+I13453+B0034 || R0040+E0240+I13453+B0034
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|-
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|'''Promoter Screening''' || E0240+I13453+B0034+C0012 ||E0240+I13453+B0034+C0051 || E0240+I13453+B0034+C0040
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|-
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|'''Wild Type Controls''' || R0010+E0240+I13453+B0034+C0012 || R0051+E0240+I13453+B0034+C0051 || R0040E0240+I13453+B0034+C0040
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|}

Revision as of 21:44, 16 August 2011

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--Monday 8/15/11--


We got our sequences from last week in and checked them to make sure they looked good. Not all of the parts were correct, but we are about a day or two from being done with construction! Here is a table of which parts are completed and which parts we need to complete in order to finish construction.

LacIc1TetR
Repressor Screening*R0010+E0240+I13453+B0034R0051+E0240+I13453+B0034 *R0040+E0240+I13453+B0034
Promoter ScreeningE0240+I13453+B0034+C0012E0240+I13453+B0034+C0051E0240+I13453+B0034+C0040
Wild Type Controls*R0010+E0240+I13453+B0034+C0012 R0051+E0240+I13453+B0034+C0051*R0040E0240+I13453+B0034+C0040


' LacI c1TetR
Repressor Screening R0010+E0240+I13453+B0034 R0051+E0240+I13453+B0034 R0040+E0240+I13453+B0034
Promoter Screening E0240+I13453+B0034+C0012 E0240+I13453+B0034+C0051 E0240+I13453+B0034+C0040
Wild Type Controls R0010+E0240+I13453+B0034+C0012 R0051+E0240+I13453+B0034+C0051 R0040E0240+I13453+B0034+C0040