Team:BU Wellesley Software/Notebook/TraciNotebook
From 2011.igem.org
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Given the lack of success of our transformations with ligations, I made a new batch of competent cells following the Anderson protocol using ''E. coli'' TOP10 cells. Over 200 tubes of 200uL aliquots of cells were prepared and frozen at -80C. | Given the lack of success of our transformations with ligations, I made a new batch of competent cells following the Anderson protocol using ''E. coli'' TOP10 cells. Over 200 tubes of 200uL aliquots of cells were prepared and frozen at -80C. | ||
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+ | Doug, Swapnil, Suma and I also had a teleconference with Josh Gilmore to discuss using invertases in our iGEM project this summer and to get his expert opinion on the invertase design scheme we came up with. | ||
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I ordered 96-well plates for the robot (Eppendorf Twin Tec Full Skirted PCR Plates) and 6 more boxes of chemically competent cells from Bioline in the hopes that they will last through the end of the iGEM project. | I ordered 96-well plates for the robot (Eppendorf Twin Tec Full Skirted PCR Plates) and 6 more boxes of chemically competent cells from Bioline in the hopes that they will last through the end of the iGEM project. | ||
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+ | |style="font-family: helvetica, arial, sans-serif;font-size:1em;color:#ea8828;"|<h5>WEEK 8: 7/25-7/29/2011</h5> | ||
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+ | I met with Swapnil and Evan, a graduate student, to discuss our plans for characterizing the simple devices the iGEM team is building using the FACS machine. | ||
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Revision as of 16:48, 16 August 2011
Wet Lab Team Notebook - Traci Haddock
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