Team:EPF-Lausanne/Notebook/August2011

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(Tuesday, 16 August 2011)
(Tuesday, 16 August 2011)
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Alessandro proceeded with the protocol started the day before from Henrike to make competent cells: the BL21 (DE3) which will be used for the T7-lys plasmid since these bacteria have the T7 polymerase gene under the control of Plac (they overexpress LacI).
Alessandro proceeded with the protocol started the day before from Henrike to make competent cells: the BL21 (DE3) which will be used for the T7-lys plasmid since these bacteria have the T7 polymerase gene under the control of Plac (they overexpress LacI).
Unfortunately the PCR purification gave us bad results for the T7-lysis which is at very low concentration. pSB3C5 instead has an high concentration.
Unfortunately the PCR purification gave us bad results for the T7-lysis which is at very low concentration. pSB3C5 instead has an high concentration.
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Henrike suggested, to have an higher yeld, to dilute your PCR products 4-fold before starting the PCR purification protocol.
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Alessandro will PCR purify the previous succesfully amplified T7-RFP products.
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Revision as of 14:18, 16 August 2011