Team:Freiburg/Notebook/15 August

From 2011.igem.org

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===NAME OF YOUR EXPERIMENT: Ligation===
===NAME OF YOUR EXPERIMENT: Ligation===
 +
'''Investigators: Sophie'''<br/>
continue from experiment: Cloning (12.8.11)<br/>
continue from experiment: Cloning (12.8.11)<br/>
Project name: inducible promoter for pbd<br/>
Project name: inducible promoter for pbd<br/>

Revision as of 10:35, 16 August 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME


blue light receptor

Transformation

Investigators: Sophie

Transformation of ligated parts:

  • LovTAP
  • Not-Gate
  • T3 vector


stored in incubator at 37°C.

red light receptor

PCR for Cph8

Investigators:Julia


after protocol from Uppsala. PCR-Mixture for one Reaction:

For a 50 µl reaction use


32,5µl H20
10µl 5x Phusion Buffer
2.5µl Primer fw
2.5µl Primer dw
1µl dNTPs
1µl DNA-Template
0.5 µl Phusion (add in the end)

Primer used:
cph8 Prefix: TTCGAATTCGCGGCCGCTTCTAGATGGCCACCACCGTACAA
cph8 Suffix: CCGCTACTAGTATTATTACCCTTCTTTTGTCATGCCCT

PCR program:
Temp. Time,Nr of cycles
98° 5' 1x
98° 30 10x
65° 30
72° 1'15
98° 30 20x
72° 1'15
72° 7' 1x
4° ∞

Template: Voights plasmid (10x dilution)
Amplicon: 2235 bp

Elongation time Phusion 67,05 sec

Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

NAME OF YOUR EXPERIMENT: Ligation

Investigators: Sophie
continue from experiment: Cloning (12.8.11)
Project name: inducible promoter for pbd
samples stored in Ruediger's box

NAME OF YOUR EXPERIMENT: Transformation

Investigators: Sophie
continue from experiment: Ligation
Project name: inducible promoter for pbd
samples stored in incubator

                       

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