Team:Colombia/Notebook
From 2011.igem.org
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* Biobrick 2 presented no colonies. | * Biobrick 2 presented no colonies. | ||
* Colonies from bricks 1, 3, 4 and 5 were stinged. | * Colonies from bricks 1, 3, 4 and 5 were stinged. | ||
- | Task: To make LB medium (15x25mL) | + | Task: |
+ | To make LB medium (15x25mL) | ||
===July 6=== | ===July 6=== | ||
* Biobricks 1, 3 and 4 were twice plated. | * Biobricks 1, 3 and 4 were twice plated. | ||
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* LB medium was prepared (15x25mL). | * LB medium was prepared (15x25mL). | ||
* Liquid LB was prepared (400mL). | * Liquid LB was prepared (400mL). | ||
- | Task: To add ampiciline and tetracicline to the boxes. | + | Task: |
+ | To add ampiciline and tetracicline to the boxes. | ||
Claim the liquid LB | Claim the liquid LB | ||
Scrape .... | Scrape .... | ||
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* Kanamicine resistence plasmid: 0.2 optic density. | * Kanamicine resistence plasmid: 0.2 optic density. | ||
* Direct inoculation x 2 and C(-) MgCl2. | * Direct inoculation x 2 and C(-) MgCl2. | ||
- | Task: Print electroporation protocol. | + | Task: |
+ | Print electroporation protocol. | ||
Ask Juan D. Olarte about the inoculation in coffee. | Ask Juan D. Olarte about the inoculation in coffee. | ||
Minipreps for confirmation of 1, 3 and 4. | Minipreps for confirmation of 1, 3 and 4. |
Revision as of 04:08, 16 August 2011
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Daily iGemWork
Here you can find our daily work in the Lab!
June
June 30:
- Biobricks 1, 2, 3, 4 and 5 were resuspended
- Miniprep Solutions (I, II and III) were prepared.
July
July 1
- Biobricks 1, 2, 3, 4 and 5 were resuspended.
July 5
- Biobrick 2 presented no colonies.
- Colonies from bricks 1, 3, 4 and 5 were stinged.
Task: To make LB medium (15x25mL)
July 6
- Biobricks 1, 3 and 4 were twice plated.
- Brick 5 didn't grow up.
- We've added Ampiciline (3.75mL) and Kanamicine (1.875mL) to the boxed from the previous day.
- LB medium was prepared (15x25mL).
- Liquid LB was prepared (400mL).
Task: To add ampiciline and tetracicline to the boxes. Claim the liquid LB Scrape .... Sting biobricks 2 and 5 (no clons) Pick up the tubes with Merceditas
July 7
- Clons 2 and 5 didn't work out.
- Clons 1, 3 and 4 were planted in LB liquid.
- E. Coli inoculation in Coffee
- Kanamicine resistence plasmid: 0.2 optic density.
- Direct inoculation x 2 and C(-) MgCl2.
Task: Print electroporation protocol. Ask Juan D. Olarte about the inoculation in coffee. Minipreps for confirmation of 1, 3 and 4. Competent cells for clones 2 and 5.
July 8
- Minipreps for 1, 3 and 4 were made.