Team:Colombia/Notebook

From 2011.igem.org

(Difference between revisions)
(Daily iGemWork)
(Daily iGemWork)
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* Biobrick 2 presented no colonies.
* Biobrick 2 presented no colonies.
* Colonies from bricks 1, 3, 4 and 5 were stinged.
* Colonies from bricks 1, 3, 4 and 5 were stinged.
-
Task: To make LB medium (15x25mL)
+
      Task:  
 +
      To make LB medium (15x25mL)
===July 6===
===July 6===
* Biobricks 1, 3 and 4 were twice plated.
* Biobricks 1, 3 and 4 were twice plated.
Line 19: Line 20:
* LB medium was prepared (15x25mL).
* LB medium was prepared (15x25mL).
* Liquid LB was prepared (400mL).
* Liquid LB was prepared (400mL).
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Task: To add ampiciline and tetracicline to the boxes.
+
      Task:  
 +
      To add ampiciline and tetracicline to the boxes.
       Claim the liquid LB
       Claim the liquid LB
       Scrape ....
       Scrape ....
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* Kanamicine resistence plasmid: 0.2 optic density.
* Kanamicine resistence plasmid: 0.2 optic density.
* Direct inoculation x 2 and C(-) MgCl2.
* Direct inoculation x 2 and C(-) MgCl2.
-
Task: Print electroporation protocol.
+
      Task:  
 +
      Print electroporation protocol.
       Ask Juan D. Olarte about the inoculation in coffee.
       Ask Juan D. Olarte about the inoculation in coffee.
       Minipreps for confirmation of 1, 3 and 4.
       Minipreps for confirmation of 1, 3 and 4.

Revision as of 04:08, 16 August 2011

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Contents

Daily iGemWork

Here you can find our daily work in the Lab!

June

June 30:

  • Biobricks 1, 2, 3, 4 and 5 were resuspended
  • Miniprep Solutions (I, II and III) were prepared.

July

July 1

  • Biobricks 1, 2, 3, 4 and 5 were resuspended.

July 5

  • Biobrick 2 presented no colonies.
  • Colonies from bricks 1, 3, 4 and 5 were stinged.
     Task: 
     To make LB medium (15x25mL)

July 6

  • Biobricks 1, 3 and 4 were twice plated.
  • Brick 5 didn't grow up.
  • We've added Ampiciline (3.75mL) and Kanamicine (1.875mL) to the boxed from the previous day.
  • LB medium was prepared (15x25mL).
  • Liquid LB was prepared (400mL).
     Task: 
     To add ampiciline and tetracicline to the boxes.
     Claim the liquid LB
     Scrape ....
     Sting biobricks 2 and 5 (no clons)
     Pick up the tubes with Merceditas

July 7

  • Clons 2 and 5 didn't work out.
  • Clons 1, 3 and 4 were planted in LB liquid.
  • E. Coli inoculation in Coffee
  • Kanamicine resistence plasmid: 0.2 optic density.
  • Direct inoculation x 2 and C(-) MgCl2.
     Task: 
     Print electroporation protocol.
     Ask Juan D. Olarte about the inoculation in coffee.
     Minipreps for confirmation of 1, 3 and 4.
     Competent cells for clones 2 and 5.

July 8

  • Minipreps for 1, 3 and 4 were made.