Team:Freiburg/Notebook/15 August

From 2011.igem.org

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cph8 Suffix:  CCGCTACTAGTATTATTACCCTTCTTTTGTCATGCCCT<br/>
cph8 Suffix:  CCGCTACTAGTATTATTACCCTTCTTTTGTCATGCCCT<br/>
<br/>
<br/>
 +
PCR program:  <br/> Temp. Time,Nr of cycles <br/>
 +
98° 5' 1x <br/>
 +
98° 30'' 10x <br/>
 +
65° 30'' <br/>
 +
72° 1'15'' <br/>
 +
98° 30'' 20x <br/>
 +
72° 1'15'' <br/>
 +
72° 7' 1x <br/>
 +
4° ∞ <br/>
 +
<br/>
 +
 +
Template: Voights plasmid (10x dilution)<br/>
 +
Amplicon: 2235 bp<br/>
 +
 +
Elongation time Phusion 67,05 sec
==<span style="color:orange;">Lysis cassette</span>==
==<span style="color:orange;">Lysis cassette</span>==

Revision as of 15:49, 15 August 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME


blue light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



red light receptor

PCR for Cph8

Investigators:Julia


after protocol from Uppsala. PCR-Mixture for one Reaction:

For a 50 µl reaction use


32,5µl H20
10µl 5x Phusion Buffer
2.5µl Primer fw
2.5µl Primer dw
1µl dNTPs
1µl DNA-Template
0.5 µl Phusion (add in the end)

Primer used:
cph8 Prefix: TTCGAATTCGCGGCCGCTTCTAGATGGCCACCACCGTACAA
cph8 Suffix: CCGCTACTAGTATTATTACCCTTCTTTTGTCATGCCCT

PCR program:
Temp. Time,Nr of cycles
98° 5' 1x
98° 30 10x
65° 30
72° 1'15
98° 30 20x
72° 1'15
72° 7' 1x
4° ∞

Template: Voights plasmid (10x dilution)
Amplicon: 2235 bp

Elongation time Phusion 67,05 sec

Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

NAME OF YOUR EXPERIMENT

Investigators: NAME

                       

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