Team:Wisconsin-Madison/test

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<a href="https://2011.igem.org/Team:Wisconsin-Madison/projectoverview">Project</a> >>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/projectoverview">Overview</a>,
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/ethanol">Ethanol Sensor</a>,
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/alkane">Alkane Sensor</a>,
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/bmc">Microcompartment</a>
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Alkane Sensor
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In the production of large chain alkanes for biofuel production, it is crucial for there to be a rapid and accurate diagnostic for comparing production rates in engineered strains of <i>E. coli.</i> To develop an alkane biosensor, <a href="https://2011.igem.org/Team:Wisconsin-Madison/genes">genes</a> from <i>Pseudomonas putida</i> and <i>Alcanivorax borkumensis</i> were isolated and constructed into a pair of <a href="https://2011.igem.org/Team:Wisconsin-Madison/plasmids">plasmids</a> which code for proteins that bind to alkanes and then induce a promoter upstream of a red fluorescent protein. After gathering data at several different concentrations of alkane, a linear regression was produced, allowing for the quantification of an unknown ethanol concentration in a media based upon the level of fluorescence. 
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Learn more about: <a href="https://2011.igem.org/Team:Wisconsin-Madison/genes">genes</a>
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Revision as of 15:26, 15 August 2011

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