Team:Wisconsin-Madison/test

From 2011.igem.org

(Difference between revisions)
Line 1: Line 1:
<html>
<html>
-
    <style>
 
-
        hr
 
-
        { border: none;
 
-
            border-top: 1px #000000 solid;
 
-
        }
 
-
 
-
        #globalWrapper
 
-
        { //background: url('https://static.igem.org/mediawiki/2011/a/a5/Bg2.jpg') top center;
 
-
            //width: 100%;
 
-
            //position: fixed;
 
-
            //margin-left: auto;
 
-
            //margin-right: auto;
 
-
            background-color: #222423;
 
-
        }
 
-
 
-
        #top-section
 
-
        { display: none;
 
-
        }
 
-
 
-
        #sddm
 
-
        { margin: 0;
 
-
            padding: 0;
 
-
        z-index: 30
 
-
        }
 
-
 
-
        #sddm li
 
-
        { margin: 0;
 
-
            padding: 0;
 
-
            list-style: none;
 
-
            float: left;
 
-
            font: bold 11px arial
 
-
        }
 
-
 
-
        #sddm li a
 
-
        { display: block;
 
-
            margin: 0 1px 0 0;
 
-
            padding: 4px 30px;
 
-
            width: 101px;
 
-
            background: #222423;
 
-
          <html>
 
     <style>
     <style>
         hr  
         hr  
Line 195: Line 155:
<li><a href="https://2011.igem.org/Team:Wisconsin-Madison/umad" onmouseover="mopen('m1')" onmouseout="mclosetime()">Home</a>
<li><a href="https://2011.igem.org/Team:Wisconsin-Madison/umad" onmouseover="mopen('m1')" onmouseout="mclosetime()">Home</a>
<div id="m1" onmouseover="mcancelclosetime()" onmouseout="mclosetime()">
<div id="m1" onmouseover="mcancelclosetime()" onmouseout="mclosetime()">
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison">Main</a>
+
<a href="https://2011.igem.org/Team:Wisconsin-Madison/umad">Main</a>
<a href="https://2011.igem.org/Team:Wisconsin-Madison/whatisigem">What is iGEM?</a>
<a href="https://2011.igem.org/Team:Wisconsin-Madison/whatisigem">What is iGEM?</a>
</div>
</div>
Line 216: Line 176:
</div>
</div>
</li>
</li>
-
<li><a href="https://2011.igem.org/Team:Wisconsin-Madison/calender" onmouseover="mopen('m4')" onmouseout="mclosetime()">Notebook</a>
+
<li><a href="https://2011.igem.org/Team:Wisconsin-Madison/notebook" onmouseover="mopen('m4')" onmouseout="mclosetime()">Notebook</a>
<div id="m4" onmouseover="mcancelclosetime()" onmouseout="mclosetime()">
<div id="m4" onmouseover="mcancelclosetime()" onmouseout="mclosetime()">
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/protocols">Protocols</a>
+
<a href="https://2011.igem.org/Team:Wisconsin-Madison/notebookprotocols">Protocols</a>
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/calender">Calender</a>
+
<a href="https://2011.igem.org/Team:Wisconsin-Madison/notebookcalender">Calender</a>
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/references">References</a>
+
<a href="https://2011.igem.org/Team:Wisconsin-Madison/notebookexperiements">Experiment</a>
 +
<a href="https://2011.igem.org/Team:Wisconsin-Madison/notebookreferences">References</a>
</div>
</div>
</li>
</li>
Line 234: Line 195:
</li>
</li>
</ul>
</ul>
-
</center> //margin-right: auto;
+
</center>
-
            background-color: #222423;
+
-
        }
+
-
        #top-section
+
<center>
-
        { display: none;
+
    <img src="https://static.igem.org/mediawiki/2011/a/aa/Logo_v2.2.jpg"; style="position:absolute; left:-10px; top:-10px"/>
-
        }
+
</center>
-
        #sddm
 
-
        { margin: 0;
 
-
            padding: 0;
 
-
        z-index: 30
 
-
        }
 
-
        #sddm li
+
<p><br> <p><br> <p><br> <p><br> <p><br> <p><br> <p><br> <p><br>
-
        { margin: 0;
+
<!--COOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOONTENT STARTS HUR-->
-
            padding: 0;
+
-
            list-style: none;
+
-
            float: left;
+
-
            font: bold 11px arial
+
-
        }
+
-
        #sddm li a
+
<a href="https://2011.igem.org/Team:Wisconsin-Madison/projectoverview">Project</a> >>
-
        { display: block;
+
<a href="https://2011.igem.org/Team:Wisconsin-Madison/projectoverview">Overview</a>,
-
            margin: 0 1px 0 0;
+
<a href="https://2011.igem.org/Team:Wisconsin-Madison/ethanol">Ethanol Sensor</a>,
-
            padding: 4px 30px;
+
<a href="https://2011.igem.org/Team:Wisconsin-Madison/alkane">Alkane Sensor</a>,
-
            width: 101px;
+
<a href="https://2011.igem.org/Team:Wisconsin-Madison/bmc">Microcompartment</a>
-
            background: #222423;
+
<p>
-
            color: #FFF;
+
-
            text-align: center;
+
-
            text-decoration: none
+
-
        }
+
-
        #sddm li a:hover
+
<table border="0" cellspacing="4" cellpadding="0"><tr><td>
-
        { background: #434343
+
-
        }
+
-
        #sddm div
+
<img src="https://static.igem.org/mediawiki/2011/7/78/Alks_picture.jpg" align="right" width="350">
-
        { position: absolute;
+
<strong><font size="3">
-
            visibility: hidden;
+
Alkane Sensor
-
            margin: 0;
+
</font></strong><p>
-
            padding: 0;
+
-
            background: #EAEBD8;
+
-
            border: 1px solid #5970B2
+
-
        }
+
-
        #sddm div a
 
-
        { position: relative;
 
-
            display: block;
 
-
            margin: 0;
 
-
            padding: 5px 10px;
 
-
            width: auto;
 
-
            white-space: nowrap;
 
-
            text-align: left;
 
-
            text-decoration: none;
 
-
            background: #eeeeee;
 
-
            color: #535353;
 
-
            font: 11px arial
 
-
        }
 
-
        #sddm div a:hover
+
In the production of large chain alkanes for biofuel production, it is crucial for there to be a rapid and accurate diagnostic for comparing production rates in engineered strains of <i>E. coli.</i> To develop an alkane biosensor, <a href="https://2011.igem.org/Team:Wisconsin-Madison/genes">genes</a> from <i>Pseudomonas putida</i> and <i>Alcanivorax borkumensis</i> were isolated and constructed into a pair of <a href="https://2011.igem.org/Team:Wisconsin-Madison/plasmids">plasmids</a> which code for proteins that bind to alkanes and then induce a promoter upstream of a red fluorescent protein. After gathering data at several different concentrations of alkane, a linear regression was produced, allowing for the quantification of an unknown ethanol concentration in a media based upon the level of fluorescence. 
-
        { background: #4e4e4e;
+
<p><br>
-
            color: #FFF
+
Learn more about: <a href="https://2011.igem.org/Team:Wisconsin-Madison/genes">genes</a>
-
        }
+
-
       
+
-
        #footerfake
+
-
        {  position:absolute;
+
-
            bottom: -55px;
+
-
            left:-5px;
+
-
            width:100%;
+
-
            // Height of the footer
+
-
            height:1px;
+
-
            //background:#222423;
+
-
}
+
-
        #fcoli
 
-
        {  position:absolute;
 
-
            bottom: -40px;
 
-
            left:770px;
 
-
            // Height of the footer
 
-
            height:1px;
 
-
            //background:#222423;
 
-
        }
 
-
       
 
-
        table
 
-
        {
 
-
        table-layout: fixed;
 
-
        margin-left: 15px;
 
-
        margin-right: 15px
 
-
        }
 
-
    </style>
 
-
            <!--STYLE ENDS HURRRRRRRRRR-->
 
-
   
 
-
    <head>
 
-
        <script type="text/javascript">
 
-
            var timeout = 500;
 
-
            var closetimer = 0;
 
-
            var ddmenuitem = 0;
 
-
           
 
-
            // open hidden layer
 
-
            function mopen(id)
 
-
            { // cancel close timer
 
-
                mcancelclosetime();
 
-
                // close old layer
 
-
                if(ddmenuitem) ddmenuitem.style.visibility = 'hidden';
 
-
               
 
-
                // get new layer and show it
 
-
                ddmenuitem = document.getElementById(id);
 
-
                ddmenuitem.style.visibility = 'visible';
 
-
            }
 
-
           
 
-
            // close showed layer
 
-
        function mclose()
 
-
            {  if(ddmenuitem) ddmenuitem.style.visibility = 'hidden';
 
-
            }
 
-
           
 
-
            // go close timer
 
-
            function mclosetime()
 
-
            {  closetimer = window.setTimeout(mclose, timeout);
 
-
            }
 
-
            // cancel close timer
+
</td></tr></table>
-
            function mcancelclosetime()
+
-
            {  if(closetimer)
+
-
                {window.clearTimeout(closetimer);
+
-
                closetimer = null;
+
-
                }
+
-
            }
+
-
            // close layer when click-out
 
-
            document.onclick = mclose;
 
-
        </script>
 
-
    </head>
 
-
   
 
-
    <!--BODYYYYYYYYYYYYY STARTS HUR-->
 
-
     <body>
+
<p><br> <p><br>
 +
    <div id="footerfake">
 +
        <img src="https://static.igem.org/mediawiki/2011/3/3e/Medium_footer.jpg">
 +
     </div>
-
<center style="position:absolute; left:2px; top:210px">
+
    <div id="fcoli">  
-
<ul id="sddm">
+
        <img src="https://static.igem.org/mediawiki/2011/e/ee/Mini_ecoli.png">
-
<li><a href="https://2011.igem.org/Team:Wisconsin-Madison/umad" onmouseover="mopen('m1')" onmouseout="mclosetime()">Home</a>
+
     </div>
-
<div id="m1" onmouseover="mcancelclosetime()" onmouseout="mclosetime()">
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison">Main</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/whatisigem">What is iGEM?</a>
+
-
</div>
+
-
</li>
+
-
<li><a href="https://2011.igem.org/Team:Wisconsin-Madison/projectoverview" onmouseover="mopen('m2')" onmouseout="mclosetime()">Project</a>
+
-
<div id="m2" onmouseover="mcancelclosetime()" onmouseout="mclosetime()">
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/projectoverview">Overview</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/ethanol">Ethanol Sensor</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/alkane">Alkane Sensor</a>
+
-
                <a href="https://2011.igem.org/Team:Wisconsin-Madison/directedevolution">Directed Evolution</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/bmc">Microcompartment</a>
+
-
</div>
+
-
</li>
+
-
<li><a href="https://2011.igem.org/Team:Wisconsin-Madison/teamoverview" onmouseover="mopen('m3')" onmouseout="mclosetime()">Team</a>
+
-
<div id="m3" onmouseover="mcancelclosetime()" onmouseout="mclosetime()">
+
-
                <a href="https://2011.igem.org/Team:Wisconsin-Madison/teamoverview">Overview</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/teammembers">Members</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/teamadvisors">Advisors</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/teamsponsors">Sponsors</a>
+
-
</div>
+
-
</li>
+
-
<html>
+
-
    <style>
+
-
        hr
+
-
        { border: none;
+
-
            border-top: 1px #000000 solid;
+
-
        }
+
-
 
+
-
        #globalWrapper
+
-
        { //background: url('https://static.igem.org/mediawiki/2011/a/a5/Bg2.jpg') top center;
+
-
            //width: 100%;
+
-
            //position: fixed;
+
-
            //margin-left: auto;
+
-
            //margin-right: auto;
+
-
            background-color: #222423;
+
-
        }
+
-
 
+
-
        #top-section
+
-
        { display: none;
+
-
        }
+
-
 
+
-
        #sddm
+
-
        { margin: 0;
+
-
            padding: 0;
+
-
        z-index: 30
+
-
        }
+
-
 
+
-
        #sddm li
+
-
        { margin: 0;
+
-
            padding: 0;
+
-
            list-style: none;
+
-
            float: left;
+
-
            font: bold 11px arial
+
-
        }
+
-
 
+
-
        #sddm li a
+
-
        { display: block;
+
-
            margin: 0 1px 0 0;
+
-
            padding: 4px 30px;
+
-
            width: 101px;
+
-
            background: #222423;
+
-
            color: #FFF;
+
-
            text-align: center;
+
-
            text-decoration: none
+
-
        }
+
-
 
+
-
        #sddm li a:hover
+
-
        { background: #434343
+
-
        }
+
-
 
+
-
        #sddm div
+
-
        { position: absolute;
+
-
            visibility: hidden;
+
-
            margin: 0;
+
-
            padding: 0;
+
-
            background: #EAEBD8;
+
-
            border: 1px solid #5970B2
+
-
        }
+
-
 
+
-
        #sddm div a
+
-
        { position: relative;
+
-
            display: block;
+
-
            margin: 0;
+
-
            padding: 5px 10px;
+
-
            width: auto;
+
-
            white-space: nowrap;
+
-
            text-align: left;
+
-
            text-decoration: none;
+
-
            background: #eeeeee;
+
-
            color: #535353;
+
-
            font: 11px arial
+
-
        }
+
-
 
+
-
        #sddm div a:hover
+
-
        { background: #4e4e4e;
+
-
            color: #FFF
+
-
        }
+
-
       
+
-
        #footerfake
+
-
        {  position:absolute;
+
-
            bottom: -55px;
+
-
            left:-5px;
+
-
            width:100%;
+
-
            // Height of the footer
+
-
            height:1px;
+
-
            //background:#222423;
+
-
}
+
-
 
+
-
        #fcoli
+
-
        {  position:absolute;
+
-
            bottom: -40px;
+
-
            left:770px;
+
-
            // Height of the footer
+
-
            height:1px;
+
-
            //background:#222423;
+
-
        }
+
-
       
+
-
        table
+
-
        {
+
-
        table-layout: fixed;
+
-
        margin-left: 15px;
+
-
        margin-right: 15px
+
-
        }
+
-
 
+
-
    </style>
+
-
            <!--STYLE ENDS HURRRRRRRRRR-->
+
-
   
+
-
 
+
-
    <head>
+
-
        <script type="text/javascript">
+
-
            var timeout = 500;
+
-
            var closetimer = 0;
+
-
            var ddmenuitem = 0;
+
-
           
+
-
            // open hidden layer
+
-
            function mopen(id)
+
-
            { // cancel close timer
+
-
                mcancelclosetime();
+
-
                // close old layer
+
-
                if(ddmenuitem) ddmenuitem.style.visibility = 'hidden';
+
-
               
+
-
                // get new layer and show it
+
-
                ddmenuitem = document.getElementById(id);
+
-
                ddmenuitem.style.visibility = 'visible';
+
-
            }
+
-
           
+
-
            // close showed layer
+
-
        function mclose()
+
-
            {  if(ddmenuitem) ddmenuitem.style.visibility = 'hidden';
+
-
            }
+
-
           
+
-
            // go close timer
+
-
            function mclosetime()
+
-
            {  closetimer = window.setTimeout(mclose, timeout);
+
-
            }
+
-
 
+
-
            // cancel close timer
+
-
            function mcancelclosetime()
+
-
            {  if(closetimer)
+
-
                {window.clearTimeout(closetimer);
+
-
                closetimer = null;
+
-
                }
+
-
            }
+
-
 
+
-
            // close layer when click-out
+
-
            document.onclick = mclose;
+
-
        </script>
+
-
     </head>
+
-
   
+
-
    <!--BODYYYYYYYYYYYYY STARTS HUR-->
+
-
 
+
-
    <body>
+
-
 
+
-
<center style="position:absolute; left:2px; top:210px">
+
-
<ul id="sddm">
+
-
<li><a href="https://2011.igem.org/Team:Wisconsin-Madison/umad" onmouseover="mopen('m1')" onmouseout="mclosetime()">Home</a>
+
-
<div id="m1" onmouseover="mcancelclosetime()" onmouseout="mclosetime()">
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison">Main</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/whatisigem">What is iGEM?</a>
+
-
</div>
+
-
</li>
+
-
<li><a href="https://2011.igem.org/Team:Wisconsin-Madison/projectoverview" onmouseover="mopen('m2')" onmouseout="mclosetime()">Project</a>
+
-
<div id="m2" onmouseover="mcancelclosetime()" onmouseout="mclosetime()">
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/projectoverview">Overview</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/ethanol">Ethanol Sensor</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/alkane">Alkane Sensor</a>
+
-
                <a href="https://2011.igem.org/Team:Wisconsin-Madison/directedevolution">Directed Evolution</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/bmc">Microcompartment</a>
+
-
</div>
+
-
</li>
+
-
<li><a href="https://2011.igem.org/Team:Wisconsin-Madison/teamoverview" onmouseover="mopen('m3')" onmouseout="mclosetime()">Team</a>
+
-
<div id="m3" onmouseover="mcancelclosetime()" onmouseout="mclosetime()">
+
-
                <a href="https://2011.igem.org/Team:Wisconsin-Madison/teamoverview">Overview</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/teammembers">Members</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/teamadvisors">Advisors</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/teamsponsors">Sponsors</a>
+
-
</div>
+
-
</li>
+
-
<li><a href="https://2011.igem.org/Team:Wisconsin-Madison/calender" onmouseover="mopen('m4')" onmouseout="mclosetime()">Notebook</a>
+
-
<div id="m4" onmouseover="mcancelclosetime()" onmouseout="mclosetime()">
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/protocols">Protocols</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/calender">Calender</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/references">References</a>
+
-
</div>
+
-
</li>
+
-
<li><a href="https://2011.igem.org/Team:Wisconsin-Madison/outreach" onmouseover="mopen('m5')" onmouseout="mclosetime()">Outreach</a>
+
-
<div id="m5" onmouseover="mcancelclosetime()" onmouseout="mclosetime()">
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/scienceolympiad">Science Olympiad</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/reuposterSession">REU Poster Session</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/socialmedia">Social Media</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/presentations">Presentations</a>
+
-
</div>
+
-
</li>
+
-
<li><a href="https://2011.igem.org/Team:Wisconsin-Madison/safety" onmouseover="mopen('m6')" onmouseout="mclosetime()">Safety</a>
+
-
</li>
+
-
</ul>
+
-
</center> <a href="https://2011.igem.org/Team:Wisconsin-Madison/calender">Calender</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/references">References</a>
+
-
</div>
+
-
</li>
+
-
<li><a href="https://2011.igem.org/Team:Wisconsin-Madison/outreach" onmouseover="mopen('m5')" onmouseout="mclosetime()">Outreach</a>
+
-
<div id="m5" onmouseover="mcancelclosetime()" onmouseout="mclosetime()">
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/scienceolympiad">Science Olympiad</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/reuposterSession">REU Poster Session</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/socialmedia">Social Media</a>
+
-
<a href="https://2011.igem.org/Team:Wisconsin-Madison/presentations">Presentations</a>
+
-
</div>
+
-
</li>
+
-
<li><a href="https://2011.igem.org/Team:Wisconsin-Madison/safety" onmouseover="mopen('m6')" onmouseout="mclosetime()">Safety</a>
+
-
</li>
+
-
</ul>
+
-
</center>
+

Revision as of 15:24, 15 August 2011









Project >> Overview, Ethanol Sensor, Alkane Sensor, Microcompartment

Alkane Sensor

In the production of large chain alkanes for biofuel production, it is crucial for there to be a rapid and accurate diagnostic for comparing production rates in engineered strains of E. coli. To develop an alkane biosensor, genes from Pseudomonas putida and Alcanivorax borkumensis were isolated and constructed into a pair of plasmids which code for proteins that bind to alkanes and then induce a promoter upstream of a red fluorescent protein. After gathering data at several different concentrations of alkane, a linear regression was produced, allowing for the quantification of an unknown ethanol concentration in a media based upon the level of fluorescence.


Learn more about: genes



Retrieved from "http://2011.igem.org/Team:Wisconsin-Madison/test"