Team:Wisconsin-Madison/test

From 2011.igem.org

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<center style="position:absolute; left:2px; top:210px">
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<ul id="sddm">
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<li><a href="https://2011.igem.org/Team:Wisconsin-Madison/umad" onmouseover="mopen('m1')" onmouseout="mclosetime()">Home</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/umad">Main</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/whatisigem">What is iGEM?</a>
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<li><a href="https://2011.igem.org/Team:Wisconsin-Madison/projectoverview" onmouseover="mopen('m2')" onmouseout="mclosetime()">Project</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/projectoverview">Overview</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/ethanol">Ethanol Sensor</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/alkane">Alkane Sensor</a>
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                <a href="https://2011.igem.org/Team:Wisconsin-Madison/directedevolution">Directed Evolution</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/bmc">Microcompartment</a>
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                <a href="https://2011.igem.org/Team:Wisconsin-Madison/teamoverview">Overview</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/teammembers">Members</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/teamadvisors">Advisors</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/teamsponsors">Sponsors</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/notebookprotocols">Protocols</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/notebookcalender">Calender</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/notebookexperiements">Experiment</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/notebookreferences">References</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/scienceolympiad">Science Olympiad</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/reuposterSession">REU Poster Session</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/socialmedia">Social Media</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/presentations">Presentations</a>
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<li><a href="https://2011.igem.org/Team:Wisconsin-Madison/safety" onmouseover="mopen('m6')" onmouseout="mclosetime()">Safety</a>
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<p><br> <p><br> <p><br> <p><br> <p><br> <p><br> <p><br> <p><br>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/projectoverview">Project</a> >>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/projectoverview">Overview</a>,
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/ethanol">Ethanol Sensor</a>,
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/alkane">Alkane Sensor</a>,
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/bmc">Microcompartment</a>
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<img src="https://static.igem.org/mediawiki/2011/7/78/Alks_picture.jpg" align="right" width="350">
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<strong><font size="3">
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Alkane Sensor
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</font></strong><p>
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In the production of large chain alkanes for biofuel production, it is crucial for there to be a rapid and accurate diagnostic for comparing production rates in engineered strains of <i>E. coli.</i> To develop an alkane biosensor, <a href="https://2011.igem.org/Team:Wisconsin-Madison/genes">genes</a> from <i>Pseudomonas putida</i> and <i>Alcanivorax borkumensis</i> were isolated and constructed into a pair of <a href="https://2011.igem.org/Team:Wisconsin-Madison/plasmids">plasmids</a> which code for proteins that bind to alkanes and then induce a promoter upstream of a red fluorescent protein. After gathering data at several different concentrations of alkane, a linear regression was produced, allowing for the quantification of an unknown ethanol concentration in a media based upon the level of fluorescence. 
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<p><br>
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Learn more about: <a href="https://2011.igem.org/Team:Wisconsin-Madison/genes">genes</a>
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Revision as of 15:19, 15 August 2011