Team:Wisconsin-Madison/test

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<li><a href="https://2011.igem.org/Team:Wisconsin-Madison/umad" onmouseover="mopen('m1')" onmouseout="mclosetime()">Home</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/whatisigem">What is iGEM?</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/notebookexperiements">Experiment</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/notebookreferences">References</a>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/protocols">Protocols</a>,
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/projectoverview">Project</a> >>
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/projectoverview">Overview</a>,
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/ethanol">Ethanol Sensor</a>,
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/alkane">Alkane Sensor</a>,
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<a href="https://2011.igem.org/Team:Wisconsin-Madison/bmc">Microcompartment</a>
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Schedule
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Alkane Sensor
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<ul><li> Week of May 10th – all DNA requests (strains, plasmids, primers, synthesis) must be submitted
 
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</li><li> Week of July 15th – presentation outline and first draft of background section will be presented at the weekly meeting
 
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</li><li> Week of August 15th – First draft of research presentation will be given in weekly meeting
 
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</li><li> August 16 - Project Description
 
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</li><li> August 27th – T-shirt design should be finalized so we can submit the order.
 
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</li><li> September 15th – Second draft of research presentation will be given in weekly meeting, poster outline should be completed.
 
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</li><li> September 20th - Project Abstract, Track, Team Roster, attendance fees due to HQ
 
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</li><li> October 27th - <b>Judging Form</b>, <b>PROJECT &amp; PARTS SUBMISSION</b>, <b>DNA SUMBMISSION</b>
 
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</li><li> October 15th – Final Poster is submitted for printing.
 
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Notebooks
 
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<ul><li> <a href="/Team:Wisconsin-Madison/notebook/Sarah" title="Team:Wisconsin-Madison/patrick">Patrick's Notebook</a>
 
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</li><li> <a href="/Team:Wisconsin-Madison/mary">Mary's Notebook</a>
 
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</li><li> <a href="/Team:Wisconsin-Madison/Eric">Eric's Notebook</a>
 
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</li><li> <a href="/Team:Wisconsin-Madison/xiong">Xiong's Notebook</a>
 
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</li><li> <a href="/Team:Wisconsin-Madison/Yaming">Yaming's Notebook</a>
 
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</li><li> <a href="/Team:Wisconsin-Madison/Ian">Ians's Notebook</a>
 
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</li><li> <a href="/Team:Wisconsin-Madison/kenneth">Kenneth's Notebook</a>
 
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In the production of large chain alkanes for biofuel production, it is crucial for there to be a rapid and accurate diagnostic for comparing production rates in engineered strains of <i>E. coli.</i> To develop an alkane biosensor, <a href="https://2011.igem.org/Team:Wisconsin-Madison/genes">genes</a> from <i>Pseudomonas putida</i> and <i>Alcanivorax borkumensis</i> were isolated and constructed into a pair of <a href="https://2011.igem.org/Team:Wisconsin-Madison/plasmids">plasmids</a> which code for proteins that bind to alkanes and then induce a promoter upstream of a red fluorescent protein. After gathering data at several different concentrations of alkane, a linear regression was produced, allowing for the quantification of an unknown ethanol concentration in a media based upon the level of fluorescence. 
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Learn more about: <a href="https://2011.igem.org/Team:Wisconsin-Madison/genes">genes</a>

Revision as of 15:15, 15 August 2011









Project >> Overview, Ethanol Sensor, Alkane Sensor, Microcompartment

Alkane Sensor

In the production of large chain alkanes for biofuel production, it is crucial for there to be a rapid and accurate diagnostic for comparing production rates in engineered strains of E. coli. To develop an alkane biosensor, genes from Pseudomonas putida and Alcanivorax borkumensis were isolated and constructed into a pair of plasmids which code for proteins that bind to alkanes and then induce a promoter upstream of a red fluorescent protein. After gathering data at several different concentrations of alkane, a linear regression was produced, allowing for the quantification of an unknown ethanol concentration in a media based upon the level of fluorescence.


Learn more about: genes



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