Team:Caltech/Week 9
From 2011.igem.org
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Transformed cells with I732017 and K323089 for some source of lacZ<br/> | Transformed cells with I732017 and K323089 for some source of lacZ<br/> | ||
Re-assembly of DDT gene using PIPE cloning and Gibson, transformed into different cells. | Re-assembly of DDT gene using PIPE cloning and Gibson, transformed into different cells. | ||
+ | Begin time-dependent biofilm optimization experiment with crystal violet stain. | ||
===Results=== | ===Results=== | ||
I732019 and I732005 plates had no colonies<br/> | I732019 and I732005 plates had no colonies<br/> | ||
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CPCR of pNT002 colonies had no insert bands<br/> | CPCR of pNT002 colonies had no insert bands<br/> | ||
DDT assembly transformations showed colonies<br/> | DDT assembly transformations showed colonies<br/> | ||
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==August 11== | ==August 11== | ||
Colony PCR of DDT gene colonies<br/> | Colony PCR of DDT gene colonies<br/> | ||
Transformation of I732005, I732017, I732019 in new XL-10 cells<br/> | Transformation of I732005, I732017, I732019 in new XL-10 cells<br/> | ||
PCR of pSB4A5, pSB3K3, pNT002 insert, pNT003 insert. Tried PCA to assemble inserts more specifically.<br/> | PCR of pSB4A5, pSB3K3, pNT002 insert, pNT003 insert. Tried PCA to assemble inserts more specifically.<br/> | ||
+ | Continue time-dependent biofilm optimization experiment with crystal violet stain. | ||
===Results=== | ===Results=== | ||
[[File:DDT_assembly_cpcr.jpg|thumb|left|CPCR of DDT gene]] | [[File:DDT_assembly_cpcr.jpg|thumb|left|CPCR of DDT gene]] | ||
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</table> | </table> | ||
==August 12== | ==August 12== | ||
+ | Continue time-dependent biofilm optimization experiment with crystal violet stain. | ||
===Results=== | ===Results=== | ||
Biobrick transformation plates | Biobrick transformation plates |
Revision as of 02:13, 13 August 2011
Project |
August 8Testing for biofilms grown on 96-well plate and Erlenmeyer flasks with crystal violet ResultsBacteria-incubated well remained cystal violet stained after several rinses, indicating the presence of a biofilm, in contrast to the clear control well. Got successful amplification of 16s in LA River sample 9-9. In both gels, the controls had no bands and all other sample lanes had no bands. August 9Evaporate-centrifuge BPA sample to concentrate for GCMS ResultsI732019 and I732005 plates had no colonies. August 10Minimal media transfers ResultsI732019 and I732005 plates had no colonies August 11Colony PCR of DDT gene colonies Results
August 12Continue time-dependent biofilm optimization experiment with crystal violet stain. ResultsBiobrick transformation plates
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