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Team:Northwestern/Notebook/Protocols/CCMB80
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*10 mM KOAc pH 7.0 (10 ml of a 1M stock/L) | *10 mM KOAc pH 7.0 (10 ml of a 1M stock/L) | ||
Latest revision as of 18:14, 12 August 2011
PROJECT
RESULTS
CONSIDERATIONS
ABOUT US
NOTEBOOK
ATTRIBUTIONS
CCMB80 Buffer
- 10 mM KOAc pH 7.0 (10 ml of a 1M stock/L)
- 80 mM CaCl2.2H2O (11.8 g/L)
- 20 mM MnCl2.4H2O (4.0 g/L)
- 10 mM MgCl2.6H2O (2.0 g/L)
- 10% glycerol (100 ml/L)
- Adjust pH DOWN to 6.4 with 0.1N HCl if necessary
- Adjusting pH up will precipitate manganese dioxide from Mn containing solutions.
- Sterile filter and store at 4°C
- Slight dark precipitate appears not to affect its function
Adapted from [http://openwetware.org/wiki/TOP10_chemically_competent_cells OpenWetWare]
