Team:Caltech/Week 9
From 2011.igem.org
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Sequencing shows pNT003 and pNT002 colonies were self-ligated. But then what was the band on the cpcr gel?<br/> | Sequencing shows pNT003 and pNT002 colonies were self-ligated. But then what was the band on the cpcr gel?<br/> | ||
DDT assembly failed.<br/> | DDT assembly failed.<br/> | ||
+ | 16s gel had primer lengths, gene did not amplify.<br/> | ||
==August 10== | ==August 10== | ||
Minimal media transfers<br/> | Minimal media transfers<br/> |
Revision as of 19:13, 11 August 2011
Project |
August 8Testing for biofilms grown on 96-well plate and Erlenmeyer flasks with crystal violet ResultsBacteria-incubated well remained cystal violet stained after several rinses, indicating the presence of a biofilm, in contrast to the clear control well. Got successful amplification of 16s in LA River sample 9-9. In both gels, the controls had no bands and all other sample lanes had no bands. August 9Evaporate-centrifuge BPA sample to concentrate for GCMS ResultsI732019 and I732005 plates had no colonies. August 10Minimal media transfers ResultsI732019 and I732005 plates had no colonies
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