Team:SouthBend-Mishawaka-HS/Notebook

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(9)Added 1mL of LB (5-17-11 DG)<br>
(9)Added 1mL of LB (5-17-11 DG)<br>
(10)Plated 100 microliters and 25 microliters of transformed cells on LB-AMP (5-19-11 "crew")
(10)Plated 100 microliters and 25 microliters of transformed cells on LB-AMP (5-19-11 "crew")
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{|align="justify"
 
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
 
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|[[Image:SouthBend-Mishawaka-HS_logo.png|200px|right|frame]]
 
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
 
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|[[Image:SouthBend-Mishawaka-HS_team.png|right|frame|Your team picture]]
 
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|align="center"|[[Team:SouthBend-Mishawaka-HS | Team Example]]
 
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<!--- The Mission, Experiments --->
 
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!align="center"|[[Team:SouthBend-Mishawaka-HS/Attributions|Attributions]]
!align="center"|[[Team:SouthBend-Mishawaka-HS/Attributions|Attributions]]
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==Notebook==
 
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You should make use of the calendar feature on the wiki and start a lab notebook.  This may be looked at by the judges to see how your work progressed throughout the summer.  It is a very useful organizational tool as well.
 

Revision as of 21:45, 19 May 2011

May

May18

(1)Poured 12 LB AMP plates:(2mg of Ampicillin to 250ml of Agar)
(2)Obtained a vial of 2 microliters of TOP-10 electrocompetant E. coli cells (44-0003/793762)
(3)Added 50 microliters of pure water from the Gemini machine
(4)Added 10 ul of water to well 6E of plate 2, which contained part F2620.
(5)Added 2 ul of the diluted DNA in 50 ul of transformation solution (50mm CaCl2, pH 6.1 BIO-RAD Transformation Solution control # 31000008916 recieved 1-17-11 GT).
(6)Incubate on ice for 5 minutes
(7)Heat shocked at 40 degrees Celcius for 50 seconds
(8)Incubated at 4 degrees Celcius for 5 minutes
(9)Added 1mL of LB (5-17-11 DG)
(10)Plated 100 microliters and 25 microliters of transformed cells on LB-AMP (5-19-11 "crew")

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