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Team:SouthBend-Mishawaka-HS/Notebook
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==May18== | ==May18== | ||
| - | Poured 12 LB AMP plates: | + | Poured 12 LB AMP plates:(2mg of Ampicillin to 250ml of Agar)<br> |
| - | + | Obtained a vial of 2 microliters of TOP-10 electrocompetant <i>E. coli</i> cells (44-0003/793762)<br> | |
| - | + | Added 50 microliters of pure water from the Gemini machine<br> | |
| - | Added 10 ul of water to well 6E of plate 2, which contained part F2620. Put 2 ul of the diluted DNA and placed in 50 ul of transformation solution (50mm CaCl2, pH 6.1). | + | Added 10 ul of water to well 6E of plate 2, which contained part F2620. Put 2 ul of the diluted DNA and placed in 50 ul of transformation solution (50mm CaCl2, pH 6.1).<br> |
| + | Incubate on ice for 5 minutes<br> | ||
| + | Heat shocked at 40 degrees Celcius for 50 seconds<br> | ||
| + | Incubated at 4 degrees Celcius for 5 minutes<br> | ||
| + | Added 1mL of LB (5-17-11 DG)<br> | ||
{|align="justify" | {|align="justify" | ||
Revision as of 21:39, 19 May 2011
May
May18
Poured 12 LB AMP plates:(2mg of Ampicillin to 250ml of Agar)
Obtained a vial of 2 microliters of TOP-10 electrocompetant E. coli cells (44-0003/793762)
Added 50 microliters of pure water from the Gemini machine
Added 10 ul of water to well 6E of plate 2, which contained part F2620. Put 2 ul of the diluted DNA and placed in 50 ul of transformation solution (50mm CaCl2, pH 6.1).
Incubate on ice for 5 minutes
Heat shocked at 40 degrees Celcius for 50 seconds
Incubated at 4 degrees Celcius for 5 minutes
Added 1mL of LB (5-17-11 DG)
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Notebook
You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.
