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Team:Cornell/Week 1
From 2011.igem.org
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Rockyshell92 (Talk | contribs) (→Tuesday) |
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:::3. Quantify the samples using nanodrop. | :::3. Quantify the samples using nanodrop. | ||
:::4. Digest 1μg of DNA sample for 2hours in the 37°C waterbath for 2hours. | :::4. Digest 1μg of DNA sample for 2hours in the 37°C waterbath for 2hours. | ||
| - | :::5. | + | :::5. PCR clean up the samples and quantify using nanodrop. |
::'''PartII''' | ::'''PartII''' | ||
:::1. Miniprepping the overnight culture using Qaigen Kit. | :::1. Miniprepping the overnight culture using Qaigen Kit. | ||
| Line 25: | Line 25: | ||
:::4. Add CIAP to the backbone and put in the 50°C waterbath for 5minutes. | :::4. Add CIAP to the backbone and put in the 50°C waterbath for 5minutes. | ||
:::5. Run the samples on the agarose gel. | :::5. Run the samples on the agarose gel. | ||
| + | :::6. Cut out the band of right size, gel purify and quantify with nanodrop. | ||
==Wednesday== | ==Wednesday== | ||
Revision as of 17:26, 10 August 2011
Results |
Protocol |
Notebook |
Parts Submitted
Week 1 | Week 2 - 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Week 20 | Week 21 |
June 5th - June 11th
Sunday
Monday
Tuesday
- CUgem bootcamp training session (day 1)
- PartI
- 1. PCR setup
- 2. Run an agarose gel and Gel purify the samples using Qaigen Kit.
- 3. Quantify the samples using nanodrop.
- 4. Digest 1μg of DNA sample for 2hours in the 37°C waterbath for 2hours.
- 5. PCR clean up the samples and quantify using nanodrop.
- PartII
- 1. Miniprepping the overnight culture using Qaigen Kit.
- 2. Quantify the samples using nanodrop.
- 3. Digest 1μg of DNA sample for 2hours in the 37°C waterbath for 2hours.
- 4. Add CIAP to the backbone and put in the 50°C waterbath for 5minutes.
- 5. Run the samples on the agarose gel.
- 6. Cut out the band of right size, gel purify and quantify with nanodrop.
- PartI
Wednesday
- Weill Hall labspace introduction by Dr.Archer
- Cugem bootcamp training session (day 2)
Thursday
- Cugem bootcamp training session (day 3)
Friday
- Team meeting
- Organized the new lab space in Weill.
- Ordered the materials needed for the labwork.
