"
Team:Cambridge/Protocols/Making Competent Cells
From 2011.igem.org
(Difference between revisions)
(→In Other Bacteria) |
(→Theory) |
||
| Line 6: | Line 6: | ||
===Theory=== | ===Theory=== | ||
| - | While none of the mechanisms by which cells take up new DNA have been well characterised, the conditions required in order for the mechanism to take place are well documented. This generally requires placing the cells under a specific type of stress which causes this to take place. | + | While none of the mechanisms by which cells take up new DNA have been well characterised, the conditions required in order for the mechanism to take place are well documented. This generally requires placing the cells under a specific type of stress which causes this to take place. Once prepared, competent cells can be stored indefinitely at -80 degrees celsius. |
===Practice=== | ===Practice=== | ||
Revision as of 16:02, 10 August 2011
Loading...
Making Competent Cells
Competent cells are those which will take up extra-cellular DNA and express it. This technique is central to synthetic biology.
Theory
While none of the mechanisms by which cells take up new DNA have been well characterised, the conditions required in order for the mechanism to take place are well documented. This generally requires placing the cells under a specific type of stress which causes this to take place. Once prepared, competent cells can be stored indefinitely at -80 degrees celsius.
Practice
In E.Coli
TODO: talk to PJ about this.
In other bacteria
The conditions required for other bacteria to be competent vary, indeed, some bacteria are naturally competent.
Safety
The safety implication of the procedure.
