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Team:Amsterdam
From 2011.igem.org
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<h1>Abstract</h1> | <h1>Abstract</h1> | ||
| - | + | <i>Escherichia coli</i>'s optimal growth temperature is 37°C. When decreasing the temperature the growth rate of the bacterium decreases rapidly. Once it reaches 7 °C, growth of wild type '<i>E. coli</i> is impossible.<br> | |
| - | In our project, we will try to increase the cold tolerance of | + | In our project, we will try to increase the cold tolerance of <i>E. coli</i>, allowing it to grow faster at temperatures below 37°C. At the end of our project, we expect to be able to culture our bacteria at very low temperatures, possibly even at or below freezing point.<br> |
| - | The approach we use involves heterologous expression of proteins in the DH5α | + | The approach we use involves heterologous expression of proteins in the DH5α <i>E. coli</i> strain. Out of different (combinations of) synthesized genes and parts submitted by previous iGEM teams, we will create cold resistance BioBricks – also known as CryoBricks. <br> |
These CryoBricks will be valuable to both fundamental and applied research. One of the many applications is replacing antibiotic resistance with cold resistance as a selection property in plasmid backbones. They can also be useful in various projects of other iGEM teams, and may open new avenues for heterologous expression of heat-sensitive proteins.<br> | These CryoBricks will be valuable to both fundamental and applied research. One of the many applications is replacing antibiotic resistance with cold resistance as a selection property in plasmid backbones. They can also be useful in various projects of other iGEM teams, and may open new avenues for heterologous expression of heat-sensitive proteins.<br> | ||
<br> | <br> | ||
| - | <h1> News</h1> | + | <h1>News</h1> |
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Revision as of 15:28, 10 August 2011
iGEM team Amsterdam 2011
Welcome to the wiki of team iGEM Amsterdam 2011! On this site, you can find out everything about the team that represents Amsterdam in this year's edition of the iGEM competition, as well as our project: icE. coli. You will find answers to questions like...- Who are the team members?
- What are they working on?
- Who's paying for all this?
Please take a look around, and be sure to contact us with any questions you are left with!
Abstract
Escherichia coli's optimal growth temperature is 37°C. When decreasing the temperature the growth rate of the bacterium decreases rapidly. Once it reaches 7 °C, growth of wild type 'E. coli is impossible.In our project, we will try to increase the cold tolerance of E. coli, allowing it to grow faster at temperatures below 37°C. At the end of our project, we expect to be able to culture our bacteria at very low temperatures, possibly even at or below freezing point.
The approach we use involves heterologous expression of proteins in the DH5α E. coli strain. Out of different (combinations of) synthesized genes and parts submitted by previous iGEM teams, we will create cold resistance BioBricks – also known as CryoBricks.
These CryoBricks will be valuable to both fundamental and applied research. One of the many applications is replacing antibiotic resistance with cold resistance as a selection property in plasmid backbones. They can also be useful in various projects of other iGEM teams, and may open new avenues for heterologous expression of heat-sensitive proteins.
News
Lorem ipsum dolor sit amet, consectetuer adipiscing elit. Aenean commodo ligula eget dolor. Aenean massa. Cum sociis natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus. Donec quam felis, ultricies nec, pellentesque eu, pretium quis, sem. Nulla consequat massa quis enim. Donec pede justo, fringilla vel, aliquet nec, vulputate eget, arcu. In enim justo, rhoncus ut, imperdiet a, venenatis vitae, justo. Nullam dictum felis eu pede mollis pretium. Integer tincidunt. Cras dapibus. Vivamus elementum semper nisi. Aenean vulputate eleifend tellus. Aenean leo ligula, porttitor eu, consequat vitae, eleifend ac, enim. Aliquam lorem ante, dapibus in, viverra quis, feugiat a, tellus. Phasellus viverra nulla ut metus varius laoreet. Quisque rutrum. Aenean imperdiet. Etiam ultricies nisi vel augue. Curabitur ullamcorper ultricies nisi. Nam eget dui.
Updates
- 22/09/11 - Wikifreeze: All of our pages have been finalized!
- 08/09/11 - The BioBricks pages are undergoing a major overhaul!
- 06/09/11 - Over the last weeks all pages have received updates. Be sure to check them all!
- 10/08/11 - The Description has been updated!
- 27/07/11 - The Notebook page has been restructured!
- 26/07/11 - The Students page has been updated!
- 19/07/11 - Several meetings and lab journal entries placed onto notebook
- 13/07/11 - The Project Description and Safety pages have been updated!
- 08/07/11 - Several meetings placed onto notebook
- 06/07/11 - Added several lab journal entries
- 05/07/11 - Updated several bios and notebook
- 29/06/11 - Several meetings placed onto notebook
- 28/06/11 - Many small layout changes made
- 27/06/11 - Several meetings placed onto notebook
- 24/06/11 - Added notebook calendar
- 23/06/11 - Fixed sponsor template
- 21/06/11 - Remaining protocols added
- 20/06/11 - First protocols added
- 17/06/11 - Photos added at lab environment
- 15/06/11 - Created footer
- 15/06/11 - Created all pages
- 12/06/11 - Created first version of header
- 02/06/11 - Temporary wiki online
