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Team:Freiburg/Notebook/9 August
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'''Investigators: Sophie''' | '''Investigators: Sophie''' | ||
| - | + | ====3A assembly==== | |
The last cloning of Theo and Jakob produced one clone with GFP-plastic-binding-domain (GFP-pbd) inside but without Promoter-Ribosome-binding site (PR). So I tried again to combine the GFP-pbd with PRs via 3a assenbly. I used two different PRs (4 and 6) with different RBS binding strenght. | The last cloning of Theo and Jakob produced one clone with GFP-plastic-binding-domain (GFP-pbd) inside but without Promoter-Ribosome-binding site (PR). So I tried again to combine the GFP-pbd with PRs via 3a assenbly. I used two different PRs (4 and 6) with different RBS binding strenght. | ||
Name of the samples: | Name of the samples: | ||
| - | ε5, | + | ε5 (GFP-pbd), |
pSB1A3, | pSB1A3, | ||
PR4, | PR4, | ||
PR6. | PR6. | ||
stored in "Minipreps, verdaut"-box | stored in "Minipreps, verdaut"-box | ||
| + | |||
| + | ====Ligation==== | ||
| + | I ligated ε5 (GFP-pbd) to PR4 or PR6 and to the Amp-Vector. Therefore I once took the ratio 3:1 insert to vector and once the ration 1:1 insert to vector. | ||
{{:Team:Freiburg/Templates/footer}} | {{:Team:Freiburg/Templates/footer}} | ||
Revision as of 10:20, 10 August 2011
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