Team:Freiburg/Notebook/9 August
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new 3A assenbly with Amp Vector | new 3A assenbly with Amp Vector | ||
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The last cloning of Theo and Jakob produced one clone with GFP-plastic-binding-domain (GFP-pbd) inside but without Promoter-Ribosome-binding site (PR). So I tried again to combine the GFP-pbd with PRs via 3a assenbly. I used two different PRs (4 and 6) with different RBS binding strenght. | The last cloning of Theo and Jakob produced one clone with GFP-plastic-binding-domain (GFP-pbd) inside but without Promoter-Ribosome-binding site (PR). So I tried again to combine the GFP-pbd with PRs via 3a assenbly. I used two different PRs (4 and 6) with different RBS binding strenght. | ||
Name of the samples: | Name of the samples: |
Revision as of 10:14, 10 August 2011