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Team:Caltech/Week 9
From 2011.igem.org
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BPA sample was too diluted for GCMS; must concentrate first. | BPA sample was too diluted for GCMS; must concentrate first. | ||
==August 9== | ==August 9== | ||
| - | Evaporate-centrifuge BPA sample to concentrate for GCMS | + | Evaporate-centrifuge BPA sample to concentrate for GCMS<br/> |
| - | Create glycerol stocks of experimental ''E. coli'' strain MG-1655 for biofilm experiments | + | Create glycerol stocks of experimental ''E. coli'' strain MG-1655 for biofilm experiments<br/> |
| - | Did 16s sequencing experiments with a Master Mix. | + | Did 16s sequencing experiments with a Master Mix. <br/> |
| + | CPCR of more pNT002 colonies with VF2 and VR primers.<br/> | ||
| + | Retransform I732005 and I732019 with top ten cells. Plated on kan plates and amp plates since plasmid has resistance to both.<br/> | ||
| + | ===Results=== | ||
| + | I732019 and I732005 plates had no colonies.<br/> | ||
| + | Sequencing shows pNT003 and pNT002 colonies were self-ligated. But then what was the band on the cpcr gel?<br/> | ||
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Revision as of 03:19, 10 August 2011
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Project |
August 8Testing for biofilms grown on 96-well plate and Erlenmeyer flasks with crystal violet ResultsBacteria-incubated well remained cystal violet stained after several rinses, indicating the presence of a biofilm, in contrast to the clear control well.
Got successful amplification of 16s in LA River sample 9-9. In both gels, the controls had no bands and all other sample lanes had no bands. August 9Evaporate-centrifuge BPA sample to concentrate for GCMS ResultsI732019 and I732005 plates had no colonies.
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